
marybeth pysz
senior principal research scientist, pharmacology
About
- PhD-level Research Scientist with 10+ years experience in cancer research - Variety of skills in vivo, in vitro, and ex vivo biology, including molecular imaging, molecular and cell biology, and microbiology - Interests: diagnosing and/or therapeutic treatment of cancer. - Specialties: In vivo molecular Imaging (US, PET/CT, BLI), small animal handling, mammalian cell culture & cell-based assays, protein & DNA/RNA expression analyses, microscopy, cloning & expression manipulations (virus or chemical transfection, overexpression/silencing), pharmacokinetics.
united states
san bruno
pharmaceuticals
microscopy, molecular imaging, cell culture, in vivo, in vitro, western blotting, transfection, cancer, small animal imaging, facs, mouse surgery and injection, cell signaling, qpcr, immunostaining, cryosection, molecular cloning and expression, viral expression, northern blotting, sirna/shrna
Experience

internship
west valley nuclear services
Responsible for databasing radiation safety records (Paradox database system)

senior principal research scientist, pharmacology
abbvie
* Group Leader: Pharmacology and in vivo imaging for site * Manages Pharmacology group activities: team of 8 people * In vivo efficacy, PK, and tolerability study design, timeline management, and execution in mice * Led lab startup and biological application development for in vivo imaging * Supervises PK bioanalysis execution and data analysis for mouse PK studies * Pharmacology reports in support of IND applications * Orthotopic cancer xenograft mouse models * Project team support (e.g., data analyses; presentations) and strategy * Previous roles: * GLP/non-GLP PK/TK analysis and reporting for toxicology studies in mice, rats, and cynomolgus monkeys

internship
praxair
Converted plant monitoring data from MS-DOS to Windows based program. Designed Visual Basic program in Microsoft Excel to size and cost water chillers.

research scientist
stanford university school of medicine
* Lab management: responsible for ordering and supply management * Mentored and collaborated with coworkers on various lab projects * Managed projects involving several collaborations with other labs across Stanford University * Independent research focus studying molecular ultrasound imaging strategies for early detection/diagnosis of and therapeutic treatment monitoring of cancer * Subcutaneous and orthotopic xenograft, and transgenic mouse models of cancer * Working knowledge of bioluminescence, PET, and CT imaging.

postdoctoral research scientist
stanford university school of medicine
* 2+ year fellowship in the Stanford Molecular Imaging Scholars Program * Worked with PI on grant applications * Authored/co-authored 14 publications & 1 book chapter in span of 3.5 years * Poster and oral presentations of work given at World Molecular Imaging Congress (WMIC) and AACR meetings

postdoctoral research scientist
roswell park cancer institute
* 3 year Ruth L. Kirschstein postdoctoral fellowship awardee * Investigated the role of protein kinase C signaling in regulation of cyclin D1 expression in normal and neoplastic intestine. * Authored/co-authored 6 publications * Presented work in posters at AACR meetings and department seminars * in vitro skills including * overexpression & knockdown techniques using adenovirus & lentivirus expansion & transduction, * siRNA transient transfection of cultured cells, * cell line culture, * expression analyses: western & northern blot analysis, qPCR * longitudinal analysis of pharmacological treatments affecting cell signaling pathways * flow cytometry analysis of apoptosis and cell cycle
Education
university at buffalo
chemical engineering
Minors in Math, Computer Science"]
north carolina state university
chemical engineering
Minor in Biotechnology","- Growth of and characterization of differential gene expression of biofilm formation and heat shock response by hyperthermophilic archae and bacteria","- Authored/co-authored 6 publications and 1 book chapter"]
marybeth pysz's Contact Information
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