Mario Morken

Head of Business Development at Exosome Diagnostics

Ion Torrent™ Product lines responsible for: The Ion Personal Genome Machine™ (PGM™) Ion OneTouch 2 System™ Ion Certified Service Provider Program

Laboratory of Francis S. Collins, Director, National Institutes of Health Functional Studies *Carried out experiments on genes implicated in diabetes risk *Participated in brainstorming meetings with Principal Investigator, supervisors, and post docs *Trained lab personnel in techniques, procedures, and use of equipment/robotics Cloning *Familiar with all steps *Worked with a variety of plasmid vectors *Familiar/Proficient in: imaging and use of fluorescent tags such GFP, Bacterial transformation, sequencing of colonies, transfection, and various DNA extraction and purifying techniques *Genome browsers and molecular biology software *Involved in the development of a Novel MALDI-TOF Mass Spec method to detect: Allele Specific expression & representation *Served as authoritative consultant to researchers on technical aspects of automated genotyping Publications, Awards, and Presentation *Distinguished Performance Award for 2008, 2009 *Attended and presented in bi-weekly lab meetings, NHGRI intramural retreat, and conferences involving other Principal Investigators from Michigan, USC, and Finland *Pathways Tuition Award *Up to date with current literature *Attended NIH campus talks, events, conferences, and courses *Rotated part time in NHGRI Technology Transfer office in 2012 *Represented NHGRI at the 2012 International BIO convention in Boston Selected Publications: Heritable Individual Specific and Allele-Specific Chromatin Signatures in Humans. Science. Apr 9 2010 -McDaniell R, {6 others}, Morken MA, Kucera KS, Battenhouse A, Keefe D, Collins FS, Willard HF, Lieb JD, Furey TS, Crawford GE, Iyer VR, Birney E. Effects of 34 Risk Loci for Type 2 Diabetes or Hyperglycemia on Lipoprotein Subclasses and Their Composition in 6,580 Non-diabetic Finnish Men. Diabetes, May 2011 -Alena Stančáková, {4 others}, Mario A. Morken, Francis S. Collins, Anne U. Jackson, Michael L. Boehnke, Johanna Kuusisto, Mika Ala-Korpela, Markku Laakso 27 current co-author publications

Responsibilities -Processed samples using different technologies for profit generating production process -Hybridization of samples using Affymetrix Gene Expression Arrays and 500K SNP arrays -Helped with Process Improvement, Training, and Alternative Platform Evaluation -Contributed to developing accurate and efficient QC methods -Implemented measures to better track samples, and track individual lot numbers of reagents being used -Trained new employees and temporary workers in all processes -Familiar with genotyping software -Created SOPs and directly worked with QA department for approval Platforms and Technologies -cDNA, IVT, SNP, and Microarray production laboratory -Responsible for large studies hybridizing thousands of samples on Affymetrix SNP & expression arrays -Isolated and extracted DNA from the sample tissue -Processed samples from isolation through hybridization on microarrays -Familiar with multiple DNA extraction techniques with and without robotics -Agilent aCGH and ABI SNPlex -Involved in converting proof of concept to a service offering for both technologies Molecular Techniques -Normalization of DNA, DNA quantitation, restriction enzyme digestion, DNA ligation, PCR, DNA purification, Fragmentation, End-labeling, hybridization, staining and scanning microarrays, etc. -cDNA and IVT reactions and magnetic bead cleanup of these reactions -Detection of DNA copy number variations across the genome using Agilent aCGH -Analysis of DNA and detection of SNPs through capillary electrophoresis using the ABI 3730 DNA analyzer

GPA: MS: 4.0 MBA: 3.85 -22 courses completed "The Promise of Personalized Medicine" February 17th, 2010 - Co-organized Event Distinguished Speakers: *Dr. Eric Green, Director, The National Human Genome Research Institute *Dr. Aravinda Chakravarti, Director, Center for Complex Genetic Disease, The Johns Hopkins University 8th Annual Kellogg Biotech & Healthcare Case Competition -January 22, 2011 -Hosted by Northwestern University Kellogg School of Business & Sponsored by Abbott -Team Leader -1 of 4 students representing Johns Hopkins -1 of 12 teams accepted, 46 teams applied from around the world 3rd Annual Wake Forest University Schools of Business Biotech Case Competition -February 24 & 25 2012 -Sponsored by Johnson & Johnson -Team Leader -1 of 5 students representing Johns Hopkins -Received Honorable Mention for: "Most Integrated Solution"