Maria Bintanel

+ Exploration of innovative technologies and methodologies relevant to biobanking. + Focus on emerging trends in biomedical research. + Operational support to key biobank and lab activities.

+ Monoclonal Antibody Development and Production: Overseeing the entire process, from upstream (cell culture, antibody expression) to downstream (purification, formulation). Ensuring efficient production and high-quality antibodies. + Experimental Planning and Execution: Designing experiments for internal projects and customer requests. Analyzing results and providing insightful reports. + Application Specialist for ICC-IF: Creating detailed product specifications. Supporting customers with technical expertise in immunocytochemistry and immunofluorescence. + Quality Assurance and Compliance: Developing and reviewing Standard Operating Procedures (SOPs). Documenting deviations to maintain compliance. + ISO 9001 Certification Process: Contributing to the company’s ISO 9001 certification. Ensuring adherence to quality standards. + LEAN Strategy Implementation: Actively participating in continuous process improvement. Applying LEAN principles to enhance efficiency + Safety and Environment: Promoting a safe work environment as an EHS Safety officer. Supporting smooth operations and compliance. + Publication Co-authorship: Contributing to scientific knowledge through one publication.

Supervised by Professor Jaakko Patrakka + Publication Co-authorship: Contributed to a research publication as a co-author. + Mouse Colony Management: Responsible for genotyping, handling, and maintaining mouse colonies. + Histological Techniques: Proficient in performing immunohistochemistry (IHC) and immunofluorescence (IF) on both paraffin-embedded and frozen tissues. + Additional Skill: Experienced in confocal microscopy. + Intercultural Environment: Collaborated effectively in a diverse and multicultural research setting.

Animal Care and Sample Collection Specialist + Mice Colony Wellness: Responsible for the overall health and well-being of mouse colonies. + Biological Sample Collection: Collecting various biological samples for research purposes. + Genotyping Biopsies: Obtaining tissue biopsies specifically for genotyping analysis. + Pairing and Plug Check: Managing breeding pairs and ensuring successful mating.

Supervised by Professor Ole Kiehn. + Mouse Colony Management: Responsible for genotyping, handling, and maintaining mouse colonies. +Cryosection of Frozen Tissue: Preparation of thin slices of frozen tissue for microscopic examination involving the use of a cryostat. + Collection of Spinal Cord Samples in a Mouse Model: Harvest of spinal cord tissue to study neurological disorders, spinal injuries or other related research utilizing a mouse model.

Supervised by Dr. Francesco Ramirez. + Publication Co-authorship: Contributed to a research publication as a co-author. + Contribution to the characterization of pathophysiological mechanisms in Marfan syndrome: Conducted pharmacological studies to explore how specific compounds or drugs impact Marfan-related processes. Investigated gene expression, protein abnormalities, and tissue changes associated with the syndrome. + Mouse Colony Management: Responsible for genotyping, handling, and maintaining mouse colonies. + Histological techniques: Harvest of aortic tissue. Proficient in performing immunohistochemistry (IHC) and immunofluorescence (IF) on both paraffin-embedded and frozen tissues. + Assisting with mouse cardiovascular ultrasounds and blood pressure measurements: Contributed to cardiovascular research by assisting with ultrasounds and blood pressure assessments in live mice. + Intercultural Environment: Collaborated effectively in a diverse and multicultural research setting.

Supervised by Prof Dr. Xavier Trepat. + Lab Manager in a multidisciplinary biophysics lab: Supervised the lab’s general maintenance by performing equipment maintenance, inventory management and ensuring a safe and efficient working environment. + Collaboration on the Project “Physical Forces Driving Collective Cell Migration: From Genes to Mechanism”: Actively contributed to the research project, which focused on understanding the physical forces involved in collective cell migration. The role included designing and conducting Molecular Biology experiments related to cell migration. The outcome of my work resulted in co-authorship of a research publication. + Intercultural Environment: Collaborated effectively in a diverse and multicultural research setting.

Supervised by Prof Dr. Joan Roselló-Catafau and Dr. Carmen Peralta Uroz. + Co-authorship of several publications. + Performed experimental surgery techniques : Normotermic ischaemia with partial hepatectomy. Liver transplantation. Small graft liver transplantation + Skilled in a wide variety of analytical techniques: Nucleic acid extraction, PCR and Real Time PCR Spectrophotometry (ELISA) and inmunoenzymatic determinations. Protein extraction and Western Blotting Histotechnology Cell culture + Intercultural Environment: Collaborated effectively in a diverse and multicultural research setting.

3 month Internship supervised by Prof Dr. Ramon Bartrons + Gained hands-on experience in a variety of techniques: Cell culture Bacterial technology: transformation and competence. Immunocytochemistry Molecular biology techniques (PCR, RNA and DNA extraction) and protein related techniques (extraction, quantification and WB). Buffer preparation.

+ Gained hands-on experience in several valuable techniques, particularly in the field of histology: Tissue Paraffin Inclusion. Microtomy. Cryosectioning. Staining Techniques. Additionally, assisted medical students during autopsies by observing, providing support, and learning from the practical experience of post-mortem examinations