Lynn Dong

Participation in various projects in concert with Discovery, Translational Research, Biomarker, Pathology and Pharmacodiagnostics for further development of the BMS Oncology/Immuno-Oncology and other therapeutic area pipelines; contributing to target discovery, lead selection, safety assessment, clinical biomarker validation, and indication selection for BMS clinical trials.

Direct and manage the Immunopathology Research and Development Core Laboratory, and oversee the operation of the Histology Core Facility in the Department of Biomedical Sciences. Responsibilities include conducting 2-4 day training sessions for undergraduates, technicians, graduate students, post-doctoral fellows, and residents in individual project based immunohistochemical (IHC) techniques including immunoperoxidase and immunofluorescence IHC, in situ hybridization, and TUNEL, in a wide variety of cells, tissues, and species. Assist research projects by conducting a review of research literature and providing consultation and instruction in IHC project design, sampling, and implementation, using the latest research methodologies and assist in interpretation of experimental results. Resulted more than 10 co-publications. Selected publications: 1)TiPARP forms nuclear condensates to degrade HIF-1α and suppress tumorigenesis. Lu Zhang, Ji Cao, Longying Dong, Hening Lin. Proc Natl Acad Sci U S A. 2020 Jun 16; 117(24): 13447–13456. 2) Nerve-Specific, Xenogeneic Extracellular Matrix Hydrogel Promotes Recovery Following Peripheral Nerve Injury. Prest TA, Yeager E, LoPresti ST, Zygelyte E, Martin MJ, Dong L, Gibson A, Olutoye OO, Brown BN, Cheetham J. J Biomed Mater Res A. 2018 Feb;106(2):450-459. 3) Generation and characterization of a monoclonal antibody against canine tissue factor. Stokol T, Daddona J, DeLeonardis C, Dong L, Wagner. Veterinary Immunology and Immunopathology [03 Jul 2015, 167(3-4):178-184] 4) Central prolactin receptor distribution and pSTAT5 activation patterns in breeding and non-breeding zebra finches (Taeniopygia guttata). Gen Comp Endocrinol. 2021 Jan 15;301:113657.

Development of a robust multiplexed G-protein couple receptor (GPCR) array technology for high throughput screening (HTS) for drug discovery. Evaluation of innovative cell culture surfaces with a variety of technologies, including HTS colorimetric, luminescent assays, and immunocytochemistry staining 11) Normalization methods for g-protein coupled receptor membrane array, Inventors: Longying Dong, Yulong Hong, Joydeep Lahiri, Fang Lai, Li Liu, Jeffrey G Lynn WO2009067174A1 WIPO (PCT). https://patentimages.storage.googleapis.com/bb/b6/d9/c0eb364ad45a7e/WO2009067174A1.pdf

Investigated the biomedical application of proprietary synthetic polymer and demonstrated significant effect of electrical stimulation on neural cell differentiation.

Worked on cDNA library construction, clone screening and sequencing, as well as analysis of gene expression pattern using RNA and in situ hybridization (ISH).

Demonstrated the importance of highly conserved C-terminal tetrapeptide to functional enzymatic tertiary structure via genetic manipulation approach. Developed and optimized a purification protocol for a native protein with FPLC-automated column chromatography, including hydrophobic, ion-exchange, size-exclusion and affinity columns. Characterized the purified protein with a variety of technologies, including SDS-PAGE, native-PAGE, Western blot, in gel activity assay, enzyme kinetic assay, substrate specificity, inhibitor regulation, Km and Ki values determination. Resulted three (3) first-authored peer-reviewed scientific publications.

worked in the field of molecular biology and protein biochemistry

Recipient of two-year scholarship from Japan. Constructed an effective expression system in vitro and highly expressed recombinant isoforms. Purified and characterized the recombinant isoforms. Furthermore, studied the effects of site-directed mutagenesis of conserved lysine residue on its enzymatic functions. Resulted three (3) first-authored scientific publications in peer-reviewed international journals.