Lucas Armitage
Senior Scientist @ LTZ Therapeutics
About
I am an immunologist with 9 years of experience in modeling immune cell biology and cell-cell interactions using primary and human pluripotent stem cell (hPSC)-derived cells. 9 years of experience using CRISPR/Cas9 for gene editing in hPSC and mammalian cell lines, including guide RNA design, homology directed template design, transfection of cells, clonal expansion, and clone screening for knockouts, edits, and maintained pluripotency. Most recently, a postdoctoral fellow working to create an isogenic, hPSC derived, thymic organoid to model cell-cell interactions during thymus and thymocyte development and eventually create transplant grade tissues and cells.
United States
San Mateo
Biotechnology
Flow Cytometry, Cytometry, Cell Based Assays, Plasmid Transformation, Cell Culture, Vector Design, Biology, Genomics, Vector Cloning, Bacmid, Western Blotting, Multi-color Flow Cytometry, Microsoft Excel, GraphPad Prism, Immunology, Reverse Transcription Polymerase Chain Reaction (RT-PCR), Method Development, Electronic Lab Notebooks, Microsoft Office, Immunofluorescence
Experience
Senior Scientist
LTZ Therapeutics
Scientist
Bristol Myers Squibb
Postdoctoral Fellow
University of Colorado
Aurora, Colorado, United States
Led a research project focused on differentiating human stem cells (iPSC/ESC) into thymic epithelial progenitor cells, hematopoietic progenitor cells, and splanchnic mesenchyme to combine into thymic organoids for use as a research model and transplant grade tissues for thymus replacement therapies and ex vivo T cell production.
Postdoctoral Research Associate
University of Florida
Graduate Research Assistant
University of Florida
PhD in Biomedical Sciences: Immunology & Microbiology. Led a research project focused on the impact of a single nucleotide polymorphism in PTPN22, associated with increased risk of Type 1 Diabetes, influences the function of macrophages and dendritic cells. Extensively utilized CRISPR/Cas9 for knocking genes out and editing single nucleotide polymorphisms in both iPSC and immortalized cell lines. Pioneered/Optimized methods for differentiating iPSC into monocytes, macrophages, and dendritic cells.
Laboratory Technician
University of Florida
Gainesville, Florida, United States
Assisted with a project focused on the role virally encoded microRNAs play in the establishment of latency of murine gammaherpesvirus 68 (MHV68). Primarily worked with the Lambda Red recombination system to edit a bacmid encoding MHV68.
Education
University of Florida
Biomedical Sciences, Immunology/Microbiology
Lucas Armitage's Contact Information
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