Lijie (LJ) Chen
Research Associate-Vaccine Development @ Bavarian Nordic
Germany
Greater Munich Metropolitan Area
Biotechnology
Cell Culture, Bioreactor, Protein Purification, SDS-PAGE, Western Blotting, PCR, Molecular Cloning, Quantitative Research, ELISA, Solidworks, Matlab, SolidWorks, Microsoft Excel, Customer Service, Communication, Aseptic Technique, Microfluidics, RStudio, FACS, DNA electrophoresis
Experience
Research Associate-Vaccine Development
Bavarian Nordic
Greater Munich Metropolitan Area
Technical Assistant-DSP Process Development
Spur Therapeutics
Greater Munich Metropolitan Area
- Developed purification processes of AAVs for gene therapy targeting rare diseases - Conducted robustness studies of AAV production downstream processes - Performed quantitative analyses of process intermediates
Master’s Thesis
Helmholtz Munich
Munich, Bavaria, Germany
- Compared and consolidated metabolic reactions in a genome-scale metabolic model of C. elegans with external databases - Performed data extraction, manipulation, and statistical analysis using R and Python
Antibody Development Intern
Roche
Penzberg, Bavaria, Germany
- Collaborated with cross-functional teams to produce research materials for the SARS-CoV-2 detection methods - Isolated mammalian PBMCs and analyzed primary immune cells - Optimized single B-Cell sorting protocols using multicolor flow cytometry
Student Research Assistant
MOLEQLAR Analytics
Greater Munich Metropolitan Area
- Optimized LC-MS sample preparation protocols and analyzed data for post-translational histone modifications - Created SOP templates and authored the first revision of SOPs
Research Intern
Universitätsklinikum Leipzig Medizinische Berufsfachschule Leipzig
Leipzig, Saxony, Germany
- Analyzed the impact of leptin on pulmonary inflammation in leptin-deficient mice models for obesity and lipodystrophy studies - Performed statistical analysis using GraphPad Prism and MS Excel
Manufacturing Associate
BioMarin Pharmaceutical Inc.
Novato, California, United States
- Operated various purification processes of recombinant proteins in cGMP cleanrooms with zero QE/deviation - Provided high-quality trainings to production staffs in a dynamic team environment - Established scaled-down models to implement continuous improvement of chromatography processes - Drafted SOPs and reviewed batch records
Undergraduate Researcher
UC Irvine
University of California, Irvine
- Received funding for designing prototypes for novel CD microfluidics technology - Designed and fabricated microfluidics chips - Published research results on the UCI Undergraduate Research Journal - Presented poster at the UCI Undergraduate Research Opportunities Program (UROP) Symposium
Process Development Intern
BioMarin Pharmaceutical Inc.
Novato, California, United States
- Investigated the impact of a specific raw material in cell production for enzyme replacement therapies - Assisted upstream production troubleshooting projects and developed upscaling plans for bioreactor inoculation with mammalian cell lines
Education
Technical University of Munich
Nutrition and Biomedicine
UC Irvine
Biomedical Engineering
Lijie (LJ) Chen's Contact Information
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