Kuo-Fu Tseng

1. Bispecific antibody and T cell engager development in cancer and autoimmune disease 2. Antibody discovery

- Develop tumor condition-activated antibody platforms (pro-antibody) - Develop Bispecific T cell Engagers (BiTEs) targeting cancer cell - Develop Immune checkpoint inhibiting antibody - Develop in vitro cell-based assay for testing the biological function of recombinant antibody prodrugs. - Optimized the protein production for in vivo testing including purity and stability.

• Investigated the motility of mitotic kinesin-14 motor proteins using single-molecule tracking fluorescence microscope • Demonstrated the effects of single motor motility on the microtubules dynamics with single molecule tracking fluorescent microscopy. • Discovered the processive minus-end-directed kinesin-14, OsKCH2 in land plants. • Discovered that KlpA, a kinesin-14 from the filamentous fungus, contains a context-dependent directional switch. • Engineered recombinant kinesin proteins with varied structural stiffness for motility or directional switch via protein structural study and modification (CD, NMR and Crystal structure). • Supervised and set up the standard characterization protocols of kinesin motility for graduate students and lab technicians.

• Taught lab sessions of Embryology and Development, Anatomy and Physiology, Genetics and Principle of Biology for undergraduate students.

• Dissecting the mechanisms of cytokinesis, the final stage of cell division, using spinning disc confocal microscope. o Demonstrated how spindle microtubules physically direct the redistribution of actin filaments into the cleavage furrow during cell division using single cell micromanipulation, microinjection and immunofluorescence techniques. o Dissecting the role of myosin-II phosphorylation in homologous recombination and chromosome segregation.

• Taught lab sessions of Principles of Biology, Biochemistry and Immunology for undergraduate students.

• Investigated the role of proteoglycan on the entry mechanism of vaccinia virus in mammalian cells. • Produced polyclone antibodies against the virus envelop proteins in Rabbit. • Screened the surface proteoglycan of host cell interacting with virus envelope proteins.

• Produced and purify tumor targeting Interluckine-2 fusion protein for the cancer immunotherapy. • Produced deglycosylated IgG via mutating the protein sequences of Fc region.