Kanyarat (Kay) Promchan, PhD

Expertise in NAb (Neutralizing Antibody Assay) and ELISpot for drug testing

Work on Research and Development for Bispecific Antibody Drug Discovery

Study on the roles of Leucine Zipper Transcription Factor-like 1 (LZTFL1), a novel cancer suppressor and an important regulator of Bardet-Biedl Syndrome protein ciliary trafficking on • Membrane trafficking of Transferrin Receptor 1 (TfR1), a novel role of LZTFL1 in regulating the cell surface TfR1 level by interacting with AP-1 and AP-2 complexes • Regulation of immune synapse formation during T cell activation modulating IL-5 release • Obesity and retina degeneration together with AP-1 mis-localization in some photoreceptor cells by using the Lztfl1 knockout mice • Regulation of actin cytoskeleton dynamics through the interaction with CFL1 resulting in the increase of CFL1 phosphorylation level by LIMK1 leading to the increase of F-actin level and regulating cell spreading, migration and invasion. • Immune synapse formation and interleukin release • CD4 expression level in response to HIV infection • Primary cilia formation and signaling transduction • Exosome release Publications 1. Promchan K and Natarajan V. (2020). Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1. PLoS One. 15:e0226298. 2. Jiang H, Promchan K, Lin BR, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V. (2016). LZTFL1 Upregulated by All-trans Retinoic Acid During CD4+ T Cell Activation Enhances IL-5 Production. Journal of immunology 196, 1081-1090. 3. Jiang J, Promchan K, Jiang H, Awasthi P, Marshall H, Harned A, Natarajan V. (2016). Depletion of BBS Protein LZTFL1 Affects Growth and Causes Retinal Degeneration in Mice. Journal of genetics and genomics. 43:381-391. 4. Promchan K, Chen D, Lockett S, Marshall H, Natarajan V. (2020). LZTFL1 regulates actin dynamics by interacting with and facilitating Cofilin 1 phosphorylation. In submission process to Journal of Cell Biology. More manuscripts are under different stages of publication

Studied on an amino acid transporter, Slc5A8, (a Na+-coupled high-affinity transporter) as a tumor suppressor in colon. Slc5A8 in colon dendritic cells was found to play an obligatory role in the suppression of IFN-gamma-producing T-cells in response to butyrate. Assisted PhD students for troubleshooting and experimental plan.

Studied on the mechanism of amino acid transporters including LAT1 and CD98hc function. The interaction of CD98hc with Annexin A2 and p11 was carried out. This interaction regulates mTORC1 signaling pathway through an increase in the phosphorylation levels of mTOR, Akt and 4E-BP1. Publications 1. Sakamoto S, Chairoungdua A, Nagamori S, Wiriyasermkul P, Promchan K, Tanaka H, Kimura T, Ueda T, Fujimura M, Shigeta Y, Naya Y, Akakura K, Ito H, Endou H, Ichikawa T, Kanai Y. (2009). A Novel Role of the C-terminus of b+,0AT in the ER-Golgi Trafficking of the rBAT-b+,0AT Heterodimeric Amino Acid tTransporter. Biochem J. 15; 417(2): 441-8. 2. Imai H, Kaira K, Oriuchi N, Shimizu K, Tominaga H, Yanagitani N, Sunaga N, Ishizuka T, Nagamori S, Promchan K, Nakajima T, Yamamoto N, Mori M, Kanai Y. (2010). Inhibition of L-type Amino Acid Transporter 1 has Antitumor Activity in Non-small Cell Lung Cancer. Anticancer Res. 30(12): 4819-28.

Studied on general Biology and Ecology