Justin Winkel
Senior Research Associate @ Ampersand Biomedicines
United States
Greater Boston
Biotechnology
Agarose Gel Electrophoresis, Mammalian Cell Culture, ELISA, Western Blotting, High-Performance Liquid Chromatography (HPLC), Antibody Sequencing , RNA Isolation, Biolayer Interferometry (BLI), Fast Protein Liquid Chromatography (FPLC), SDS-PAGE, Protein Expression, Antibody Purification, Antibody sequence analysis, Reverse Transcription Polymerase Chain Reaction (RT-PCR), DNA/RNA isolation, Plasmid Cloning
Experience

X-SIG Summer Research Intern
Gettysburg, Pennsylvania, United States
Identified sgRNA targets and cloned plasmids for the targeted CRISPR-Cas9 mediated knockout of dipeptidase genes in Drosophila melanogaster cell culture to investigate the effects of dipeptidase activity on oxidative stress responses. Trained fellow lab members in D. melanogaster maintenance and standard molecular biology techniques.

Teaching Assistant
Gettysburg, Pennsylvania, United States
As a teaching assistant for a weekly Genetics lab, I provided instructional support on topics including Mendelian Genetics, Sexual Selection in D. melanogaster, and the genetics of biochemical pathways.

Research Assistant
Gettysburg, Pennsylvania, United States
Supported the operation of the Hiraizumi lab in the study of the biochemical genetics of dipeptidases in D. melanogaster. Maintained genetic strains of D. melanogaster and monitored inventory of supplies and reagents.

X-SIG Summer Research Intern
Gettysburg, Pennsylvania, United States
Optimized in vitro assays for D. melanogaster dipeptidase activity. Isolated DNA from D. melanogaster strains to support PCR amplification and Sanger sequencing of dipeptidase genes. Characterized dipeptidase expression using SDS-PAGE and Western Blot. Maintained genetic strains of D. melanogaster and laboratory supplies and reagents.
Justin Winkel's Contact Information
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