Julien Audry

- Development, optimization, and qualification /validation of assays in bacteriology and virology, ensuring precision, linearity, accuracy, specificity, robustness, and reproducibility. - Led international on‑site deployment, traveling alone to another country/continent to support laboratory operations, troubleshoot workflows, and represent the organization externally. - Contributed to pre‑transfer activities, including method refinement, documentation alignment, and preparation of materials for future assay transfer. - Participate in assay transfer initiatives, supporting technical alignment, data review, and method troubleshooting. - Manage scientific projects, coordinating timelines, priorities, and cross‑functional interactions. - Analyze complex data sets to drive assay optimization and scientific decision‑making. - Conduct investigations to resolve deviations and improve reliability, throughput, and ergonomics. - Prepare high‑quality protocols, transfer / development / qualification / validation plans and reports and regulatory documents. - Ensure compliance with quality systems and regulatory standards in a highly controlled environment. - Collaborate with multidisciplinary teams to implement innovative solutions to technical challenges. - Mentor junior staff and contribute to a positive, high‑performing team culture. - Lead client communications, facilitate technical meetings, and maintain strong collaborative relationships.

- First‑author discovery: identified that Ccq1 restrains Mre11‑dependent nuclease activity to enable end‑protection at newly formed telomeres (S. pombe). - Built and scaled an inducible proto‑telomere (I‑SceI) model to track telomere formation and showed that heterochromatin spreads gradually and remains dynamic. - Advanced methods: co‑authored a Methods in Molecular Biology protocol describing a rapidly inducible DSB system to monitor telomere formation, DNA repair, and checkpoint activation. - Collabolation with CRCM (Marseille, France): co‑authored studies on telomere stability in quiescent cells. - Model‑reframing result (first author): demonstrated that telomeres block checkpoint activation independently of H4K20me2 depletion. - Lasting impact: work seeded multiple publications **after my departure**, highlighting durable value from my experimental systems, strains, and datasets. • Contributed tools and constructs to the field. The proto‑telomere formation system/parts are available to the community.

- PhD researcher, CRCM (Marseille) – “Telomeres & Chromatin” team led by Vincent Géli. - Thesis: Replication and maintenance of telomeres in Schizosaccharomyces pombe — RPA’s ability to prevent/resolve secondary structures (G‑quadruplexes). - First‑author discovery: showed that RPA prevents G‑rich (G4) structure formation at lagging‑strand telomeres, sustaining shelterin association and telomerase action (The EMBO Journal, 2015). - Established genetic and molecular assays in fission yeast to interrogate telomere replication stress (e.g., RPA1‑D223Y model), Rad52 recruitment, and checkpoint readouts; designed and executed multi‑technique workflows (yeast genetics, ChIP, Southern blots, microscopy). - Collaborated closely with international partners and the CRCM teams to translate mechanistic insights on telomere replication into citable, peer‑reviewed outputs. - Contributions from my PhD work supported subsequent CRCM‑linked publications. - Communicated findings via manuscripts, seminars, and lab meetings; mentored junior students on yeast telomere methods and data analysis.

Treasurer of the association of PhD students and young researchers in biology and heath science of Aix-Marseille University, France.

Role of RPA in telomere maintenance in fission yeast

Study of the toxicity of Aβ mutants implicated in alzheimer disease in Saccharomyces cerevisiae