Juliana The

Process Development Scientist at a CDMO specializing in biological products and vaccines for research and clinical applications. • Developed and executed upstream processes for R&D and GMP projects. • Optimized bioproduction of microbial and mammalian cell cultures in shake flasks, bench-scale and pilot-scale bioreactors. • Developed and executed downstream purification processes. • Authored client- and project-specific batch records and SOPs for productions. • Oversaw and carried out technology transfer from clients to R&D, and from R&D to GMP manufacturing. • Communicated project progression to internal and external stakeholders.

Antibody Scientist in an antibody platform company, carrying out workflows in support of discovery pipeline for multiple targets and collaborations with pharmaceutical partners • Generated phage display libraries of heavy chain-only antibodies from immunized transgenic mice • Designed, optimized, and performed phage library biopanning for various projects • Analyzed biopanning enrichment data, prepared panned and unpanned phage libraries for Next Generation Sequencing • Molecular cloning of antibody and phagemid constructs

Recombinant expression and protein engineering of structure-specific endonucleases for cryo-EM studies. Insect cell protein expression and optimization. Protein-protein interaction studies.

Executed drug discovery project for a novel heart failure therapeutics targeting a focal adhesion protein-protein interaction • Designed and developed a FRET-based binding assay in mammalian cells for screening chemical probes • Carried out analytical characterization of target protein-protein interaction by biolayer interferometry, thermal shift assays and DSF • Trained and supervised graduate students on carrying out and troubleshooting protein expression, purification and fluorescence polarization assays • Troubleshot and optimized protein production and purification

Structural biology of the PRC2 complex • Worked across function with the gene cloning, baculovirus protein production, crystallography and biophysical assay groups to produce highly pure protein samples for biochemical and structural characterization. Troubleshot protein purification and designed solutions. • Published two novel EED inhibitors with AbbVie and UNC. Contributed in the characterization of protein stability (thermal shift assays), enzyme inhibition assays and determination of a co-crystal structure. • Managed collaboration with pharmaceutical and academic partners on various structural biology and chemical probe projects. Presented regular updates at teleconferences and internal meetings.

Studied structural dynamics of protein S-nitrosation and investigated phosphine chemistry for development of a new proteomics-based S-nitrosothiol detection assay. • X-ray crystallography and mutation studies of thioredoxin S-nitrosation. • Characterized reactions of phosphines with S-Nitrosothiols by NMR, mass spectrometry and isotope labeling. • Mapped out SNO-modified peptides by mass spectrometry using phosphine derivatization approach.

Carried out a biochemical project for the diabetes drug discovery project. • Characterized agonist and potentiation effects of compounds and peptides on the GLP-1 receptor. • Designed and performed dose-response cell-based potency assays in high-throughput format. • Optimized potency assays using automation which resulted in identification of new potentiators in the compound library.

Multiple x-ray crystallography projects of Mycobacterium tuberculosis (Mtb) proteins and mechanistic studies. • Carried out gene cloning, protein expression, purification, crystallization, enzymatic assays and protein interaction studies. Optimized bacterial expression and purification of multiple proteins. • Characterized folding/unfolding equilibrium of DsbF protein by CD spectroscopy.