Joshua Cremin

As recognition for continued high performance and delivery of milestones and project goals, I was promoted to Principal Scientist in November 2021

Promoted to Senior scientist in September 2018 and leading a variety of formulation projects for external partners. Also responsible for routine maintenance of HPLCs.

Protein formulation scientist for a successful formulation R&D company. Skills involve a variety of HPLC analytical methods, including high-throughput screening with UPLC systems, with experience in Waters Acquity H-class and Thermo-Ultimate systems. Other techniques include tangential flow filtration (TFF), micro-flow imaging (MFI), UV-vis spectroscopy and Design of Experiments (DoE).

See "Education" Selected techniques: PCR, restiction digests, ligation, plasmid transformation. Protein expression and purification by affinity chromatography (His-tagged). Liquid chromatography mass spectrometry analysis of intact protein masses (Q-trap). X-ray crystallography and diffraction (Diamond, ESRF, SLS) Interpretting diffraction data and modelling proteins - CCP4 suite, Coot, etc.

I worked independently in the lab of my undergraduate tutor Dr. Karen Lillycrop investigating the relationship between the diet of pregnant mice and the epigenome of their newborns. Techniques: SDS-PAGE, DNA extraction, Western blotting.

PhD supervised by Professor Sir John E Walker (Nobel laureate, 1997). My project was to characterise the regulation of ATP hydrolysis of the F-ATP synthase from Caldalkalibacillus thermarum. The ATP synthase from this bacteria only acts as a synthase, unlike mitochondrial F-ATP synthases. I expressed the soluble domain of the enzyme from E. coli and purified it with affinity chromatography (His-tag). I used X-ray crystallography and molecular replacement to determine the atomic structure of the bacterial enzyme in order to identify any differences between it and mitochondrial F-ATP synthase structures. Other biochemical experiments included activity assays, BCA protein quantification assays, luciferase assays, phosphate detection assay, and thermal stability assays.

Techniques: Protein expression, purification and quantification. SDS-PAGE analysis. Circular dichroism. Solid state NMR. My final year honours project involved investigating which phospholipids were part of the annular lipid shell for the potassium crystallographically-sited activation channel (KcsA).