Jialei Duan

Senior Scientist @ Novo Nordisk

About

Google Scholar: https://scholar.google.com/citations?hl=en&user=iLi-Oh4AAAAJ GitHub: https://github.com/jlduan ● Proficient in processing, analyzing, and interpreting large-scale omics datasets, encompassing genome-wide bisulfite DNA methylation data, single-cell and bulk expression, and chromatin accessibility data obtained from mouse and human early embryos, human organoids, disease samples, and perturbed human and mouse cell lines. ● Extensive expertise in conducting large-scale single-cell perturbation assays, including Perturb-seq/Mosaic-seq, and Reprogram-seq. ● Published peer-reviewed scientific software (Bioinformatics). Skilled in working within high-performance computing environments. ● Demonstrated track record of publishing impactful scientific papers related to mammalian early embryonic development (Cell), human organoids (Nature, Cell Stem Cell), disease classification algorithms (Circulation), and single-cell perturbation assays (Molecular Cell, Cell Reports). ● Proficient in collaborating with multidisciplinary teams and deeply passionate about problem-solving.

Country

China

City

Beijing

Industry

Biotechnology

Skill

Genomics, Molecular Biology, Genetics, Sequencing, Sequence Analysis, Computational Biology, Transcriptomics, Second Generation Sequencing, DNA, Bioinformatics, qPCR, Cell Biology, Biotechnology, Biochemistry, Cell Culture, Life Sciences, Continuous Integration and Continuous Delivery (CI/CD), DevOps, AI Prompting, Artificial Intelligence (AI)

Experience

Senior Scientist

Novo Nordisk

2023-3 - Present · 3 yrs 7 mos

Research Scientist

UT Southwestern Medical Center

2019-1 - 2023-3 · 4 yrs 3 mos

Dallas/Fort Worth Area

1. Analyzed and interpreted the first complete human embryo model in the dish with single-cell genomics. 2. Multimodally profiled healthy and disease human heart samples at single-cell resolution. 3. Developed a rapid molecular diagnostic strategy to accurately determine the etiology of cardiomyopathy by leveraging machine learning algorithms. 4. Designed novel strategies to predict new transcription factors for cell fate engineering.

Postdoctoral Researcher

UT Southwestern Medical Center

2015-10 - 2018-12 · 3 yrs 3 mos

Dallas/Fort Worth Area

1. Transcriptionally profiled mouse heart at single-cell level and identified major cardiac cell types. 2. Designed novel strategies to systematically screen thousands of transcription factor (TF) cocktails for cellular reprogramming performance and as a proof of concept, reprogrammed fibroblasts to epicardial-like cells using newly identified three TFs. 3. Analyzed and interpreted single-cell resolution CRISPR-based high throughput endogenous enhancer perturbation experiments.

Postdoctoral Research Fellow

Chinese Academy of Sciences

2012-8 - 2015-9 · 3 yrs 2 mos

Beijing City, China

1. Analyzed and interpreted allele-specific single-base resolution genome-wide DNA methylation (5mC, 5hmC, 5fC) and transcriptomic data during mouse early embryonic development. 2. Designed sequencing strategies, evaluated sequencing library quality and communicated sample collection requirements, sequencing status to team members in Nanjing University. 3. Analyzed and interpreted transcriptomic and DNA methylation data to evaluate the performances of using small molecules converting human gastric epithelial cells into endodermal progenitors. 4. Established analytical strategies to compare human and mouse methylomes at single-base resolution during early embryonic development.

Scientific Research Partner

BGI Genomics

2010-12 - 2011-5 · 6 mos

Shenzhen, Guangdong, China

As a key representative of National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences in a collaborative genomic research project with BGI Shenzhen 1. Worked closely with the BGI team to establish, customize and test bioinformatic workflows for the genome/transcriptome assembly project. 2. Communicated project requirements, status, deadlines with specialists in Beijing lab and the BGI team to ensure the smooth completion of the project.