Jin-Hwan Han

• Principal Research Fellow (연구위원 / 전무) • Head up a research team to develop innovative therapeutics for patients with cancer and other chronic diseases

• Leading oncology and immuno-oncology discovery programs - Leading project teams of scientists from multiple functional groups - Co-lead: peptide-drug conjugate discovery team (screening and optimization) - Co-lead: antibody-drug conjugate discovery team (target, undisclosed) - Co-lead: immune agonist cytokine discovery team (target, undisclosed) - Lead: allogeneic innate killer cell (i.e., NK or gdT cells) therapy proof-of-concept evaluation • Leading and contributing to research collaborations with academic research groups • Personnel management: - Directing, managing, providing scientific guidance and mentorship of a research team: interns, postdocs, and junior staff scientists

• Leading and contributing to cancer immunotherapy discovery and translational research - Immune checkpoint blockade: pre-clinical mechanism of action - anti-TIGIT (MK-7684) - Immune agonist antibody: translational research, biomarker discovery - anti-CD27 (MK-5890) - Multispecifics: leading a nanobody discovery program (collaboration with Ablynx - discontinued) - Immune agonist cytokine: characterization of a clinical candidate - Antibody-drug conjugate: investigation of proof-of-concept biology • Leading and contributing to research collaborations with academic research groups • Personnel management: - Directing, managing, providing scientific guidance and mentorship of a research team: interns, postdocs, and junior staff scientists

• Contributing to cancer immunotherapy efforts - Participating in identification and characterization of clinical candidate biologics - Pre-clinical, mechanism of action studies using syngeneic tumor models in wild-type and in genetically engineered mice - Immunophenotyping with multi-color flow cytometry - Gene expression profiling - Cell-based assays - Tumor cell engineering

• Scientific fields: infectious disease, tumor immunology, immunotherapy, vaccinology, IgG-Fc biology • Immunotherapeutic approaches to chronic LCMV infection • Immunological basis of radiotherapy to cancer - PD-1-directed, combination immunotherapy - Immunophenotyping with multi-color flow cytometry - Analysis of T cells (exhausted, activated, regulatory), NK cells, myeloid cells and tumor cells • Characterization and analysis of Fc glycan of vaccine-derived IgG in human - Handling human sample (purification of lymphocytes and plasma) - Immunophenotyping of human PBMCs with multi-color flow cytometry - In vitro culture of human primary cells - Purification of antigen-specific IgG (including antigen immobilization procedure) - Antigen-specific ELISA and ELISPOT

• Scientific fields: vaccinology, B-cell repertoire, antibody (Fc) engineering • Adjuvant effects in active immunizations in mouse models - Vaccine development - Anti-tumor response • Regulation of IgG sialylation - Characterization of IgG-Fc glycosylation - Tumor cell engineering and in vivo imaging • Analysis of IgG repertoire of antigen-specific antibody (by a single-cell cloning method) - Handling human sample (purification of lymphocytes and plasma) - Immunophenotyping of human PBMCs with multi-color flow cytometry - Single-cell sorting and analysis of antigen-specific B cells for IgG repertoire analysis

• Research advisor: Dr. Arash Grakoui • Scientific fields: infectious disease, viral immunology • Investigation of the role of CD4 T cells on CTL responses to chronic hepatitis C virus (HCV) in a chimpanzee model • Immune escape mechanism and antigen evolution in chronic viral infection - CTL epitope analysis - Ex vivo T-cell functional assays • Identification of CD4 T-cell epitopes from an HCV whole genome - Generation and characterization of T-cell hybridoma - Immunization of human MHC class II transgenic mice

• Laboratory of Dr. Thereza Imanishi-Kari • Scientific fields: autoimmunity, B-cell development, innate immunity • Thesis title: Activation of RNA-specific autoreactive B cells during B cell development on a normal and an autoimmune genetic background - Molecular and cellular analysis of mouse B cells, myeloid cells, immune complex and autoantibody - Immunophenotyping by flow cytometry and molecular biology (qPCR, Southern blot, cloning) - Development of a novel bioassay : mouse interferon-alpha (IFN-α) ELISPOT assay