Jernej Horvat

Site page: www.beyondmuradistillery.com Despite the fact that we are a new and rather small distillery in Prekmurje, our copper dome rarely rests. We have gradually developed the recipe for our Slovenian London dry gin over time and our goal is to delight each gin lover. Our story evolves because of our constant curiosity and creativity as well as your trust. We are proud and happy to present you our gins and I hope you will join us in this joy!

Pilot scale development, Scale Up, NT-PAT, Bioprocess Engineering, Modelling, Data Management

Pilot Scale Development, Scale-Up, NT-PAT, Biopharmaceuticals development

-Mammalian cells -Generics -Bioprocess technology -Process Analytical Technology (PAT) -Data management

- Phospholipase C (PLC) production with industrial Pichia pastoris strain in 5L glass bioreactors - Working with glycerol de-repression promoter (methanol-free) - Assay development for PLC quantification - Proces design and running fed-batch experiments based on a specific substrate uptake rate (qs) and online biomass estimation - Upstream (media & feed preparation, bioreactor assembling, equipment calibration, autoclaving etc.) - Bioprocess analysis - Shake flask experiments - Data presentation and interpretation - Running fed-batch experiment with Escherichia coli

Assistance in a Laboratory for diagnostics.

Isolation of heterologous isotopically labelled proteins from E. coli expression system, for nuclear magnetic resonance spectroscopy studies.

Isolation and purification of Archaea proteins.

I was working on CHO cell metabolism, where main focus was on amino acids and how their availability effects cell performance in fed batch system. I explored media design and introduced additional amino acids to main feed and see how does this effects process performance regarding cell growth, specific rates and product quality.

Master thesis tittle: Development of an efficient feeding strategy for an industrial yeast strain.