Jeff Galligan

Provide technical and scientific leadership to support new product development, troubleshooting and optimization of process and analytical methods, and technical communications with customers and suppliers. Identifies and implements new methods, equipment and technologies.

Member and team lead for the generation of cell line products in upstream bioprocessing development with the highly successful protein expression platform (CHOZN®). Responsible for: • Creation of new high producing CHO-based cell line for biotherapeutic manufacturing • Design and implementation of new diagnostic processes to validate genomic integrity • Developed critical internal collaborations for end-to-end cell line development • Led product development process and alignment with multiple organizational groups including marketing, sales, integrated supply chain, procurement, etc • Developed and delivered customer-facing presentations for new products • Regular detailed reporting to senior level business executives and stakeholders

Managed newly developed marketing segment in Bioprocess Materials focused on creating products for use in RNA therapeutics. Responsible for: • Full market and financial analyses on material components used in commercial RNA-based therapeutic manufacturing • Generated financial model for new product development from investigation and review of market research • Collaboration and alignment with multiple groups including sales, integrated supply chain, procurement • Presentation to existing and new potential external customers • Regular detailed reporting to senior level business executives and stakeholders

Postdoctoral research fellow in the lab of Peter Howley studying the molecular biology of papillomaviruses, the nature and consequences of viral-host cellular protein interactions, and the mechanisms of virus-associated cancer formation. Projects and techniques included: • The first broad proteomic analysis of the giant cellular ubiquitin ligase HERC2 to identify potential cellular HERC2-interacting proteins. A subset of potential interactors were validated for binding endogenous HERC2 and a complex bioinformatic pathway analysis implicated HERC2 in intracellular protein trafficking and transport, metabolism of cellular energy, and protein translation (Galligan JT, Martinez-Noel G, et al., JPR, 2015). • Identified and delineated the architecture of a novel large molecular weight cellular protein complex containing the ubiquitin E3 ligases E6AP and HERC2, the centrosome regulator NEURL4, and the serine/threonine kinase MAPK6 (Martinez-Noel G, Galligan JT, et al., MCB, 2012). • An investigation of the role of the cellular ubiquitin ligase HERC2 in cellular growth, DNA damage, DNA repair and HPV genome replication and amplification. • Proteomic identification of HPV oncoprotein E6-dependent and independent E6AP cellular interactors in human cervical cancer cells. • These studies required proficiency with human tissue culture techniques, cloning, agarose gel electrophoresis and SDS-PAGE, stable and transient cellular transfection for expression or RNAi-mediated knockdown of cellular proteins, affinity isolation and purification of proteins, immunofluorescence, western blot, mass spectrometry, size exclusion chromatography. • Bioinformatic analyses required expertise with : o Genomatix Pathway System (http://www.genomatix.de/) o MetaCore (https://portal.genego.com/) o Ingenuity (http://www.ingenuity.com/products/ipa) o Chilibot – natural language processing (http://www.chilibot.net/)

Co-founder of group. Presidential duties included organizing and executing a regular meeting agenda, working cooperatively with and delegating responsibilities to group officers, providing progress and projections to faculty sponsor, procuring financial and material resources from university departments, establishing and maintaining connections with personnel from local, state and national ASM affiliates. As president, the chapter grew in size and established geographical and organizational connections with the potential to provide its members with significant career opportunities.

Graduate student in the lab of Jack Kennell studying the replication of a small group of linear, autonomous, viral-like genetic elements that reside in the mitochondria of certain strains of the fungal plant pathogen Fusarium oxysporum. Projects and techniques included: • Identified a protein-primed initiation mechanism for reverse transcription utilized by the pFOXC mitochondrial retroplasmids of the fungal plant pathogen Fusarium oxysporum. Characterized mechanistic differences for nucleotide preference and template specificity between the distantly related pFOXC1 and pFOXC3 plasmid-encoded reverse transcriptases (Galligan JT, Marchetti SE, and JC Kennell, Mob DNA, 2011. • Successfully collaborated with industry scientists at Sigma-Aldrich on bacterial expression of a reverse transcriptase encoded by a fungal mitochondrial plasmid. Performed expression and enzymatic analysis onsite at the Sigma-Aldrich biotechnology facility. Participated in meetings and presented results to Sigma-Aldrich research team. • Expression and enzymatic analysis of the pFOXC3 reverse transcriptase in a rabbit reticulocyte system. • These studies required proficiency with radiolabeled nucleotides, PCR, cloning, DNA and RNA analysis (isolation, hybridization, sequencing), techniques for protein expression and isolation, immunoprecipitation, agarose gel electrophoresis and SDS-PAGE.

Teaching assistant for laboratory accompanying undergraduate courses: Principles of Biology (Introductory course) General Microbiology (Junior and Senior level course) Responsibilities included laboratory preparation and organization, lesson planning and instruction, facilitating discussion, evaluation of written and oral reports, and regular meetings with faculty overseeing course.