Janina Sörmann

Group of Prof. Stephan Pless Project: - Leveraging Deep Learning to design hASIC1a modulators for stroke treatment Methods: - Machine learning tools for binder design - Automated Patch Clamp assays - Miniprotein expression and purification - Cell culture Soft skills: - Project design and co-supervision of interns, Bachelor- and Master students - Grant writing - Teaching classes and lab courses

- Drug discovery (HTS, hit-to-lead, lead optimisation, early safety assessment) - Assay development and screening on Automated Patch-Clamp and Microelectrode array (MEA) - Ion channels and transporters - Project lead and operation - Data analysis and presentation to clients

Group of Prof. Stephen J. Tucker Projects: - Defective X-Gating of TASK-1 in a Novel Channelopathy Associated with Sleep Apnea - VHH antibodies against K2P channels- toolkit and mechanistic insights Methods: Electrophysiology Molecular Biology Nanobody expression and purification Cell culture Flow cytometry Soft skills: Co-supervising DPhil students Organising 'Oxford Biological Physics Seminar' Further education: 'Strategy and Innovation' EMBA class at Saïd Business School, University of Oxford as an Ideas-to-Impact Fellow.

Project: Defective X-gating in the potassium channel TASK-1 Collabarators: Prof. Thomas Baukrowitz and Dr Marcus Schewe

Group of Prof. Ernst Bamberg Project: Underlying mechanism of ion transport in rhodopsins Methods: electrophysiology (patch clamp, two electrode voltage clamp, black lipid membrane, solid supported membrane), molecular biology (mutagenesis, PCR, DNA extraction, RNA synthesis), fluorometry, protein expression and purification, reconstitution of membrane proteins, cell culture (creating stable cell line, transient transfection), genetic code expansion with non-canonical amino acids

Project: Conformational changes in rhodopsins monitored with fluorescent non-canonical amino acids Supervisor: Prof. Rikard Blunck Methods: cut-open oocytes voltage clamp and expression of rhodopsins with non-canoical amino acids in oocytes

Two electrode voltage clamp fluorometry experiments Supervisor: Prof Thomas Friedrich

Department of Circuit Neuroscience Project: Ascending SAG neurons control sexual receptivity of Drosophila females Supervisor: Dr. Barry Dickson Methods: patch clamp in vivo (fruit flys), fruit fly genetics, confocal microscopy

Dissertation: Ion transport in Krokinobacter Eikastus Rhodopsin-2 Advisor: Prof Ernst Bamberg Interests: Gating and transport mechanism in membrane proteins, optogentic tools, protein engineering Methods: Electrophysiology (patch-clamp, two-electrode voltage clamp (fluorometry), cut-open oocytes, black lipid membrane, solid supported membrane), protein expression and purification, molecular biology, non-canonical amino acids https://www.youtube.com/watch?v=-Tmvz2iRGtA

Master’s thesis at the Max-Planck-Institute of Biophysics in Frankfurt, Department of Biophysical Chemistry Thesis: Conformational changes of channelrhodopsin probed by voltage clamp fluorometry Supervisor: Prof. Dr. Ernst Bamberg Methods: patch clamp, two electrode voltage clamp, molecular biology, fluorometry, cell culture Main subjects: Biophysics, theoretical physics (quanten physics, thermodynamics), electrophysiology, IR spectroscopy, theoretical photochemistry, visualization in biology and medicine, biophysical chemistry II, Laser - application in chemistry

Bachelor’s thesis at the Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt Thesis: Binding dynamics of aptamers Supervisor: Prof. Dr. Josef Wachtveitl Method: RNA synthesis, fluorometry, Stopped Flow spectroscopy Main subjects: theoretical physics (I,III,IV,V), experimental physics (I–III), inorganic chemistry, organic chemistry, theoretical chemistry II, physical chemistry, molecular biology, genetics, cell biology, microbiology, biochemistry, plant physiology, bioinformatics, mathematics for scientists (I–III)