James Fasano

CMC Operations Manager @ Convergent Therapeutics, Inc.

About

Experienced Scientist with a demonstrated history of working in research and biotech. Skilled in Bioreactors, Mammalian Cell Culture, Fermentation, Molecular Biology, AAV, Lenti virus; process development & scale of up AAV, LVV, and Plasmid production. Master’s Degree focused in Biomedical Engineering from University of New Haven.

Country

United States

City

Greater Boston

Industry

Biotechnology

Skill

Fermentation Process Development, Upstream Processing, Gene Therapy, Molecular Cloning, Cell Culture, Western Blotting, Troubleshooting, Research, DNA Extraction, Molecular Biology, Data Analysis, Pharmaceutical Industry, Start-ups, Process Engineering, Scientific Writing, Real-Time Polymerase Chain Reaction (qPCR), Microsoft Word, Critical Thinking, Time Management, Teamwork

Experience

CMC Operations Manager

Convergent Therapeutics, Inc.

2026-3 - Present · 7 mos

Scientist III

Thermo Fisher Scientific

2024-2 - 2026-3 · 2 yrs 2 mos

Scientist I

ElevateBio

2022-7 - 2024-2 · 1 yr 8 mos

Principal Associate Scientist

ElevateBio

2021-7 - 2022-7 · 1 yr 1 mo

Lead a team of multiple associate scientists in AAV and LV process development to create both platforms. In charge of daily viral vector PD activities including experimental design (DoE), bioreactor scale up, and data analysis. Lead tech transfers as Upstream SME.

Senior Associate Scientist

ElevateBio

2020-6 - 2021-7 · 1 yr 2 mos

I help lead the scale up work for LVV process development work. Experienced in small scale and pilot scale bioreactors as well as DOE for process optimization. In charge of handling lab inventory and ordering supplies critical for meeting project timelines.

Senior Research Associate I

Homology Medicines, Inc.

2020-1 - 2020-6 · 6 mos

Research Associate II

Homology Medicines, Inc.

2019-5 - 2020-1 · 9 mos

Helped initiate an internal Plasmid Process as well as analytical method development. Supported AAV process development from shakeflasks through scale up to 2000L bioreactor. I performed AAV vector production/process development work in CCS10s; as well as 2L, 25L, and 50L bioreactors. I support optimizing process development by carrying out independent studies and batch purifying AAV.

Upstream Research Associate

Softworld, a Kelly Company

2018-12 - 2019-5 · 6 mos

I carry out AAV vector production in CCS10s; as well as 2L and 50L bioreactors. I support optimizing process development by carrying out independent studies. I rapidly test plasmids through High-throughput Screening

Bench Scientist I

Mindlance

2018-7 - 2018-12 · 6 mos

Responsible for running experiments for process validation with CHO cells in 2L bioreactors. I maintained instruments used for sampling analysis as well as the bioreactors themselves.

Postgraduate Associate

Yale University

2017-6 - 2018-7 · 1 yr 2 mos

I am investigating the roles histones and chaperone proteins have on terminal erythroid differentiation, with a focus on enucleation. I work with mice, to obtain embryos and remove fetal livers to culture erythroid progenitors. The cells are cultured and infected via lenti virus to alter terminal differentiation, which is visualized via flow cytometry and FACS analysis using FlowJo.

Histology Technologist

Dianon Systems

2015-1 - 2017-6 · 2 yrs 6 mos

I performed the gross examination of biological specimen and dissected them, submitting sections for pathological review. I maintained graphical representations of how samples were inked in sectioned for the pathologists to reference. I was also responsible for tissue processing and embedding samples in parrafin so they could be cut into slides.