Jacqueline Burke

Biomedical Engineering

SNC Therapeutics Inc. employs a disruptive drug and nonviral gene delivery platform technology for rational immunotherapy. Our synthetic nanocarriers (SNCs) permit scalable, versatile, and controlled drug delivery to and/or transfection of diverse cell populations while minimizing toxicity and side effects.

Department of Pharmacology & Regenerative Medicine Du Lab

-Authored numerous successfully funded grants, including a Department of Defense (DOD) award of $1.5 million and an NIH R21 of $420 K -Lead a national research project for the DOD to assess the biocompatibility of a thermoresponsive citric acid-based polymer for autologous islet transplantation in pancreatitis patients using a nonhuman primate model; Tasks include collaboration with transplant surgeons and contractors, delegating tasks to senior scientist, presenting data, determining future project directions -Supported principal investigators in the launch of multi-institutional centers, including the Center for Regenerative Engineering (CARE), in pursuit of NSF ERC and NIH RM1 awards -Successfully lobbied the Graduate School to secure a budget of $200K to purchase a spectral flow cytometer, established a curriculum for instrument training and managed a core for collaborative use of the instrument -Recognized the potential threat of anti-poly(ethylene glycol) antibodies against common drugs prior to their association with the COVID-19 vaccine; Authored review article and developed mouse models to study these effects, resulting in 2 U.S. Patent Applications No. 17/071,653 (Filed 10/2020) and 63/092,019 (Conversion 10/2021) -Developed immunomodulatory nanotherapy that enables allogeneic transplant survival with less drug and fewer side effects, resulting in U.S. Patent Application No. 16/891,391 (Filed 6/2020) -Designed and authored IACUC protocols for rodent studies in accordance with ethical best practices; Negotiated revisions with veterinary staff and review committee

-Differentiated human induced pluripotent stem cells (hiPSCs) in a pulsatile flow bioreactor, resulting in vascular smooth muscle tissue with robust elastic fibers and responsiveness to chemical cues -Analyzed the use of acelluar and hiPSC-containing dextran and hyaluronic acid-based hydrogels for improved wound healing outcomes in an immunocompetent porcine model and immunodeficient diabetic murine model -Recognized inconsistencies in data collection method and implemented solutions to ensure data accuracy -Worked with a team of undergraduate students to ensure lab safety, cleanliness, and to manage inventory

• Managed process validation efforts globally for sequencing and sequencing preparation instruments • Developed software validation requirements, plans, protocols, and reports to meet requirements for FDA 21 CFR Part 11 • Communicated expectations for quality performance, continuous improvement, quality systems, and process controls infrastructure for critical sustaining parts and new products • Acquired skills in SAP and Good Documentation Practices

-Designed an auditory drug delivery completion indicator for an autoinjector device -Collaborated with a medical device engineering team to evaluate mechanical testing results, which gave rise to more patient friendly drug delivery systems -Utilized professional development events to gain exposure to technical and financial aspects of the bio-pharmaceutical industry and drug development process, including drug discovery, regulatory approval, manufacturing and patient delivery

• Collaborated in research to determine the location of Clec16a protein binding sites involved in insulitis onset • Developed skills in polymerase chain reactions and gel electrophoresis

• Analyzed data, including patient surveys, procedure statistics, and x-rays, and made recommendations for further analysis in support of the following research publication: Saad, H, MD; Baker, E, MS; Baker, K, PhD; Carlson, N, RN; Buhovecky, T, RN; Grant, A, MD; Fortin, P, MD. Meniscal Allograft Transplantation for the Treatment of Osteoarthritis of the Metatarsophalangeal Joint (MTP) of the Great Toe. • Developed skills in scientific writing, Microsoft Office, and presentation