Jacob Sun

抗体生产CDMO服务

Main projects and achievements Seahorse: Lead the fermentation process development in seasonal flu vaccine production using a microalgae system - Successfully finished the tech transfer from DSM within months - A 30 times HA activity increase was achieved in CA09 with new medium and bioprocess - Developed a CO2-stat control strategy which is ready to scale up - Developed a continuous fermentation and primary recovery process with high productivity. The process has been tested in other antigens, like CP PRN yield improvement: Collaborate with Mtech to improve upstream CP fermentation process - PRN yield was increase by 20% within 6 month - Investigated and found the role of acids in PRN production and release, resulting 2-3 times PRN improvement. A patent is being prepared. TB: - Successfully developed a high cell density fermentation process and up to 270 OD and 14g/L TB was yield - A simple and scalable profile feed process was developed to further improve the consistency of fermentation runs between batches and scales - Developed auto-induction and auto-shut down process based on online biomass analysis - Co-authored two paper published in Vaccine, and one won a Sanofi NA Science award in 2016 - Scaled up fermentation and primary recovery process to a 200L pilot scale Protein purification: Cross training in Downstream process development

Involved in developing a cell culture process for pharmaceutical protein production Investigated the effect of culture medium and feed strategy on cell growth and protein expression Prepared documents for new drug application Facilitated solving microbial fermentation problems in industrial scale

Led fermentation process development for vaccine projects To solve the problem of oxygen limitation developed a DO-stat PID control strategy by which DO was successfully controlled at 30±2% during fermentation Successfully developed a scale up model based on kLa resulting comparable fermentation performance in large scale Developed a scalable primary recovery process for Phase III clinical trial

Performed process stimulation, specified process and equipment parameters and prepared Process Flow Diagram (PFD) Guided equipment installation and trouble shot problems in start up and operation Provided technical service and presented products and processes to potential customers New product and process development - Participated in re-engineering Jet Coflow Packing Tray which has high throughput, high efficiency and low pressure drop - Designed and evaluated a process to reduce sulfur dioxide emission in power plants - Collaborated with industrial partners to develop a process generating electricity from landfill gas ISO9001 Quality identification

Successfully enriched and isolated new microorganisms capable of degrading organic pollutants from contaminated soil Investigated and optimized culture medium and growth conditions by DOE Introduced molecular based technology to analyze microbial community Developed a metabolic flux model to study PHA fermentation

Produce human Interleukin-6 in E. coli for pre-clinical test Investigated culture medium and fermentation conditions to enhance cell growth and protein expression Minimized acetic acid production in the fermentation and enhanced protein expression based on metabolic flux analysis

Studied plant cell culture and secondary metabolite release in an air-lift bioreactor Investigated the oxygen mass transfer in bioreactor