Ioannis Intzes

• Molecular cloning of novel protein constructs using diverse molecular biology and analytical techniques. • Cultivation and manipulation of mammalian cells (Expi293F). • Execute lab scale mammalian antigen expression and purification using different chromatography techniques. • Implementation and documentation of dedicated experiments according to detailed protocols and standard operating procedures as well as contribution to general maintenance of laboratories and equipment.

• Conduct microbiological assays, including sterility testing, microbial identification, and contamination assessments under ISO and GMP standards. • Perform immunochemical analyses using ELISA for pathogen and allergen quantification. • Maintain aseptic conditions for sample preparation and testing. • Interpret experimental data and present findings

• Supported senior laboratory staff in the Microbiology Lab, performing complete blood counts, coagulation tests, and blood crossmatching. • Assisted the Platoon Sergeant in managing daily operations, maintaining records, ensuring compliance with regulations, acted as a liaison between staff and soldiers and coordinating duty rosters for 70 personnel.

• Performed research on PROTAC-based strategies aimed at targeting disease-associated proteins for therapeutic drug discovery. • Utilized Fluorescence Polarization (FP) and Surface Plasmon Resonance (SPR) to investigate protein-ligand interactions. • Designed and executed site-directed mutagenesis experiments to analyze binding site dynamics. • Purified proteins using affinity, size exclusion and anion exchange chromatography on the ÄKTA start system.

• Developed experimental methods to analyze cytokine expression levels via qPCR in an animal model for Multiple Sclerosis (MS). • Constructed single-chain variable fragment (scFv) anti-CD3 expression plasmid vectors for use in MS research. • Utilized SDS-PAGE and Western blot techniques to analyze protein expression and validate experimental results. • Performed RNA and DNA isolation, PCR techniques, genotyping, cDNA synthesis, cloning, electrophoresis, protein purification, and primary cell cultures. • Managed laboratory animal colonies, including breeding, health monitoring, and organ harvesting for research.

• Conducted qPCR-based SARS-CoV-2 diagnostics, microbiological tests, and hematological and immunological analyses. • Supported quality control measures and implemented standardized protocols for sample management and equipment maintenance.

• Contributed to: Western blot, Immunofluorescence, Confocal microscopy, Cell culture (hiPSCs, hESCs)

Graduated 3rd in class. Diploma Dissertation Title: “Construction of plasmid expression vectors of scFV anti-CD3 for use in EAE and study of cytokine expression during induction.” Techniques: RNA extraction, PCR & rtPCR, RNA & DNA electrophoresis, FACS, plasmid cloning, cell culture, lab mice colony management.