Inna Zilberleyb

* Leading technology development efforts to build a multi-host semi-automated protein expression and purification platform for delivery of proteins for biochemical and structural studies.

Highlights * Responsible for successful operation and delivery of E.coli protein production workflows. Oversee the implementation of new technologies to increase throughput and enable the delivery of challenging targets. * Contribute to providing high quality protein expression systems to support diverse protein reagent requirements for Drug Discovery and Basic Research.

Highlights * Refolded, purified, and crystallized a bacterial β-barrel membrane protein; the effort resulted in a 3.5Å crystal structure to aid in a therapeutic antibody development. * Design and optimization of expression and recovery of membrane, intracellular and secreted proteins and protein complexes. * Experienced in producing isotopically labeled proteins in E.coli for NMR studies. Experienced in selenomethionine labeling of proteins in E.coli and BEVS.

Highlights * Designed and constructed a collection of expression vectors to allow high throughput subcloning, leveraging Restriction-Independent Cloning (RIC) technology. * Contributed to building high throughput small-scale protein expression and characterization platform to support SMDD. * Involved in a close collaboration with Protein Sciences Informatics to help design and maintain user-friendly applications to ensure maximum communication, efficiency, and corporate memory for protein production activities.

Assistant Research Scientist I/II, Associate Research Scientist I As part of the Genome Biochemistry Department, I expressed, purified, and characterized proteins to support assay development, HTS campaigns, crystallography studies, and immunogen production at Exelixis.

The research involved a study of closely packed α-helices with emphasis on prediction of location, type, and packing pattern of α-helices in a protein based on amino acid sequence.

The research involved a study of structure, function and biophysics of molecular motors of the myosin and kinesin families. The emphasis was on molecular mechanism of muscle contraction and force generation in the motor proteins. Additional study: structure and function of DNA motors.

Emphasis in Biochemistry