Indrani Chakravarty
Research Associate ll @ 4D Molecular Therapeutics
About
I have 3 years of cumulative lab experience in the field of Molecular Biology/Bioinformatics with extensive knowledge in tools and procedures used in the industry including CRISPR, molecular cloning, immunofluorescence assay, animal work, plant transcriptomics and Genome Annotation Project. Skills: ELISA, DNA/RNA Extraction, cDNA Synthesis, PCR/qRT-PCR, Bioanalyzer, Gel Electrophoresis, Sample Detail Record (Blood/Serum/Tissue), Good Lab Practices, Media Preparation, Aseptic techniques, Alignment (M coffee, MUSCLE, BLAST), Phylogenetics (MEGA), Expression Atlas (EBI), Genevestigator, Basic expertise in Unix, RStudio, Maintain detailed record of research study in Electronic Lab Notebook (Platform: Benchling)
United States
San Ramon
Biotechnology
ELISA, DNA Extraction, Real Time PCR, Molecular Cloning, Gel Electrophoresis, RNA, Molecular Biology, Polymerase Chain Reaction (PCR), Biology, Clinical Research
Experience

Junior Research Fellow / Research Associate
Assam, India
Performed research to Serosurveillance and association of TLR, TH1-TH2 status and viral genotypes in susceptibility and severity of Peste des petits ruminants among goats and sheep of North East India. • Collection of blood samples and serum and ELISA testing • Total RNA extraction, cDNA synthesis • PCR/RT-PCR • Expression of TLR 3/7/8 at mRNA based expression analysis by Real Time PCR.
Education

Biological science
Assessing the Role of Sucrose Signaling in the Crosstalk between Nutrient Deficiency Responses in White Lupin • Grew white lupin plants using hydroponics for validating differentially expressed genes from a recent RNA seq project by qRT-PCR • Isolated high quality total RNA from roots verified with Bioanalyzer • cDNA synthesis • Maintain detailed record of research study in Electronic Lab Notebook (Platform: Benchling)
Indrani Chakravarty's Contact Information
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