Hyeyoung Eom, PhD

• Designe and generate Naïve antibody phage display libraries for client projects • Designe antibody panning strategies • Antibody discovery using antibody phage display libraries (Fab, ScFv, and Humanized, Camelid VHH) for oncology and infectious diseases • Generate immunized camelid phage display libraries as internal technical development • Molecular biology and molecular cloning of antibody and antigens (mutagenesis, PCR, DNA sequencing, NGS prep, IgG reformating) • Prepared Power point slide for client meeting and participated/presented the data to clients • Manage supplies and inventory

• Antibody discovery using Naïve antibody phage display libraries (Fab, ScFv, and VH) for oncology and infectious diseases • Characterization of the antibody/antigen interactions (Affinity, KD, EC50, ELISA, BLI, Flow cytometry) • Characterization of antibody physicochemical properties including aggregation propensity, solubility, stability, etc • Molecular biology and molecular cloning of antibodies and antigens (in-fusion cloning, PCR, sequencing, etc) • Protein and antibody expression (prokaryotic, eukaryotic, E.coli and Expi 293, etc) • Protein and antibody purification (FPLC, affinity, SEC, IonEx, etc) • Prepared weekly report for internal meeting and data for client update

• Designed combinatorial histidine libraries to generate pH-dependent antibodies using phage display • Characterized thermodynamic properties of pH-dependent antibody interaction • Developed expertise in protein biophysics (ITC and SPR) to study thermodynamic and kinetic of antibody/antigen interactions • Molecular biology and molecular cloning (PCR, mutagenesis, gene design, library design and construction, etc) • Protein and antibody expression (prokaryotic, E.coli) • Protein and antibody purification (FPLC, Duo Flow, AKTA, affinity, SEC, IonEx, etc) • Managed and trained several undergraduates and high school students • Supervised and maintained laboratory operation including managing supplies/inventory and instruments

• Synthesized coumarin moieties and purified using column chromatography • Characterized compounds using 1H-NMR • Used ChemDraw to draw organic structures