Hui Xu

• Gene-modified DNA construct design and generation. (Including: DNA/RNA extraction, digestion. Ligation. PCR primer design, PCR, Gibson Assembly, sequencing and sequence analysis ) Phage library construction and screening. Intein-engineered enzyme validation by enzyme activity assay and western blot. • Biochemical activity assays and ELISA assays for detecting the activity of the enzyme contained in different tissues of the transgenic corn plant.

• Using Affymetrix Genechip platform to provide clinical GeneChip tests for patient’s genetic mutation diagnosis for multiple diseases, Adherence to CLIA (or GLP /GMP) regulations. SOP reviewing and editing. • Using and maintaining the different laboratory automation system such as Beckman Coulter liquid handling robot

• Generated multiple gene targeting constructs and genetically modified mouse model. Carried out gene targeting experiments by using molecular and cell biology techniques, including molecular cloning, PCR, Southern blot, Northern blot, Western blot, embryonic stem (ES) cell and other mammalian cell culture and electroporation transfection, (such as: cell proliferation and differentiation in vitro, cell line maintenance, cell thawing, cryopreservation), ES cell and inactivated fibroblast co-culture, ES cell colony selection and screening. fibroblast cell culture and feeder cell production. • Identified the candidate genes responsible for MEL (Murine Erythroleukemia) cell proliferation and differentiation by micro-array, Northern blot analysis and Western blotting approach. • Immunohistochemistry analysis for colon cancer biomarkers. • Maintained multiple genetically modified mouse lines. Analyzed mouse model via mouse dissection, mouse surgery, whole-mount embryo in-situ hybridization