Hien N.

A joint venture start-up between Maze and Alloy therapeutics. Envisioning an end to blindness by advancing antibody therapies to treat genetic ophthalmic diseases. -Model Development and Target Validation: Implement in vitro and biochemical characterization assays to dissect ANGPTL7 target, biomarkers, MOA, and PKPD for go/no-go candidate selection. - Preclinical Development: Design, plan, and monitor safety/efficacy studies across species (rodent, rabbit, pig, NHP) to guide IND-enabling studies. Establish in-house vivarium, write and maintain IACUC protocol, and execute in-house in vivo experiments ensuring compliance with regulatory guidelines and ethical standards. -Project Management: Independently direct various CRO study timelines, review/negotiate budgets, protocols, and implementation across different time zones. - Collaboration: Generate study reports, publications, data and liaison with CROs, parent companies, investors, and team. Attend/present at external conferences and site monitor visits and compile competitive intelligence.

Acquired by Grifols. Capitalizing the body’s natural regulatory and communication mechanisms by decoding the plasma proteome to target neurodegenerative and age-related diseases. -TFF2 Antibody Discovery: Run target identification and validation studies in vitro to nominate the best candidate therapies into the preclinical pipeline. -Target Validation: Design and execute in vivo PKPD and efficacy studies to investigate molecular signaling pathways, tissue regeneration, inflammation, and age-related cognitive impairment. -Report: Analyze and interpret data to propose candidate feasibility. Write up reports and present data across different levels and audiences.

Collaborate cross-functionally across company levels to transform science to medicine by dissecting the neural circuitry behind appetite, energy homeostasis, and obesity. -Pipeline: Design and execute high throughput in vivo pharmacology experiments to advance preclinical efforts in the REGN4461 (Mibavademab) pipeline. -Target Discovery: Screen targets in vivo in mechanistic biology studies to validate disease models and possible therapeutics in generalized lipodystrophy and monogenic obesity. -Champion the integration of novel in vivo and ex vivo neurobiological techniques to understand the relationship between neural circuits of metabolism. -Report: Analyze and interpret data using statistical methods. Write up reports, INDs, ELNs, posters and present data across different levels and audiences.

Work proactively with graduate students and post-doctoral researchers to examine the following research question: how does the brain integrate multi-sensory inputs, memories, and innate impulses to make decisions? This answer can be achieved by dissecting the neural circuits underlying decision-making with behavioral paradigms (psychophysics) and measuring/manipulating neurons (optogenetics, 2-photon calcium imaging, intrinsic imaging, wide-field imaging) during the decision-making process. -Optimized live-surgery techniques resulting in faster surgeries, swifter recoveries, and robust cranial windows -Directed psychophysic experiments, established novel behavior, and innovated existing behavior -Proficient in In Vivo Electrophysiology; designed novel optetrode drives for experiments -Analysis: Evaluated psychometric behavior, clustered neural data, and immunohistochemistry -Build connections with visiting scientists at weekly seminars hosted by CSHL

1. Project Involvement: Cocaine Self-Administration in Rats -Designed and Implemented Self-Administration Experiments in accordance to SOPs, IACUC, and DEA regulations -Directed timelines, fixed ratio/progressive ratio schedules, dosages, session durations, etc -Wrote Self-Administration Protocols using GS4 -Animal Care (Rat) -Housing, Habituation, Surgery, Post-Op Care, Behavior -Analyzed and Graphed Behavioral Data with Sigma Plot12 -Statistical Analysis of Addiction Escalation -Live Surgery - Jugular Vein Catheterization Surgery -Conduct peer-reviewed literature searches Present experimental data in weekly lab meetings