Harpreet Kaur, PhD

Co-chaired the graduate student symposium at Sarnia Research Park, Canada.

Thesis: Investigation of Lysozyme Refolding Using Ionic Liquids as Refolding Additives The efficacy of Imidazolium based ionic liquids as refolding additives was compared to conventional refolding. An empirical model was developed and validated by experimental results. Experimental results indicate that in comparison to the conventional refolding buffer, among different ionic liquids examined, ionic liquid ([EMIM]+ Cl) has a pronounced effect on the refolding yield and time required to refold the denatured protein. On-column refolding studies indicate that in comparison to the packed bed system, the fluidized bed ion-exchange system approach, with [EMIM]+ Cl as the elution-refolding buffer could tolerate higher concentration of denatured protein (up to 25 mg) with refolding yield ca. 90% and fractional mass recovery ca. 82%. This was due to potentially lower mass transfer limitations in the fluidized bed compared to the packed

Development of crystallization as an extraction and purification strategy for monoclonal antibodies.

Kaur, H. “Lipid and Polymer Based Oligonucleotide Delivery Systems” (2004) Oral Presentation. Recipient of Best Student award in Pharmaceutical Science and Technology (2003-04) Under-graduate Research on “Antimicrobial potential of Honey” (2002)