Hanyi Chang

Provide Nucleic Acid formulations for preclinical pharmacology studies and coordinate pre- and post-study dose formulation analysis

Supported technology transfer to CMOs

Leading role in process optimization of mRNA lipid nanoparticles (LNP) with tangential flow filtration (TFF)

Assited in process optimization of siRNA lipid nanoparticles (LNP) with tangential flow filtration (TFF)

Formulation group - Characterize LNP including particle size, pH, osmolality - Quantify LNP's content of RNA and RNA encapsulation by Ribogreen - Preparation of buffers for dialysis and TFF Biology group -In Vitro Transcription, DNA and RNA purifications, PCR, E. coli plasmid isolation, Restriction Digest, immunostaining, and cell culturing.