Haiying Li

Role Overview Design and execute cross-platform characterization strategies for antibodies and antigens, combining advanced mass spectrometry, immunoassays, and data-integrated workflows to support discovery, screening, and product development. Key Contributions >Developed high-throughput assays such as mass spectrometry, WB, IF and IP to evaluate antibody identity, specificity, affinity, and function. >Led assay development for antibody de novo sequencing, disulfide mapping, glycosylation profiling, and intact mass analysis using Bruker Q-TOF system. >Led a scalable cell display screening platform, integrating automation and Python-based titration curve reporting for streamlined data sharing. >Led IP-WB/MS assay development for mapping antibody interactions and characterizing target engagement in native and complex lysates. >Provided scientific and technical mentorship to junior colleagues, while fostering a collaborative environment across discovery, protein engineering, and assay teams. >Played a central role in platform scaling and process documentation for antibody product nomination and validation. Tools & Techniques Orbitrap QE & Fusion | Bruker Maxis Q-TOF | ELISA | SPR | SEC | SDS-PAGE | Western Blot | Flow Cytometry | Cell Display | Python | Protein Metrics | Biopharm Compass | IP-MS | Native MS | Mass Photometry

Role Overview Led translational proteomics and glycomics research focused on identifying disease biomarkers and post-injury molecular signatures. Developed mass spectrometry workflows and collaborated across clinical, academic, and analytical teams. Key Contributions >Designed and executed quantitative proteomics studies (TMT, label-free) using Orbitrap systems for discovery of protein and glycan biomarkers in urology and spinal cord injury models. >Generated the first comprehensive urinary glycan MS reference library, enabling in-depth comparisons across pediatric and adult cohorts. >Developed LC-MS-based workflows for site-specific glycan analysis, PTM profiling, and protein studies in complex tissues (bladder, kidney, liver). >Collaborated with clinicians and bioinformaticians to integrate proteomics/glycomic data into biological and disease-relevant interpretations. >Authored peer-reviewed publications and contributed to public proteomics datasets. Techniques & Tools Orbitrap QE | NanoLC-MS/MS | TMT | Label-Free Quant | Glycomics | PTM Analysis | Membrane Protein Prep | Python | R | Scaffold | Skyline | MaxQuant | Biognosys | Pathway Enrichment

Role Overview Established and led the Proteomics Core Facility, supporting research across the university through advanced mass spectrometry-based analytical services and training. Key Contributions >Set up proteomics facility, overseeing instrument installation, SOP development, and lab operations. >Led acquisition and deployment of high-resolution LC-MS systems (Waters, Thermo, Bruker, Agilent), including vendor negotiation and maintenance protocols. >Managed a team and coordinated internal and external collaborative research projects. >Supervised training programs for graduate students and scientists in proteomics workflows, data analysis, and sample preparation. >Initiated internal consultation services to support experimental design and data interpretation for faculty labs. Techniques & Tools LC-MS/MS (Orbitrap, Q-TOF) | Protein Identification | PTM Analysis | PRM | DIA | Scaffold | Biopharm Compass | MaxQuant | Bioinformatics Integration | SOP Development | Vendor Management

Focus Developed advanced mass spectrometry-based workflows for protein glycosylation analysis and post-translational modification (PTM) mapping. Conducted cross-disciplinary collaborations in glycobiology, protein engineering, and analytical chemistry. Key Contributions >Established LC-MS/MS pipelines for site-specific N-glycan profiling using Orbitrap Fusion and nanoLC platforms. >Discovered novel phosphorylated N-glycans in yeast-expressed proteins. >Collaborated with international researchers across analytical chemistry and molecular biology departments. >Presented findings at international conferences and contributed to peer-reviewed publications. >Served as a lecturer at the EMBO Mass Spectrometry Course (2012), training early-career scientists in proteomic technologies. Skills & Tools NanoLC-MS/MS | Glycomics | PTM Analysis | Protein Expression | Orbitrap Fusion | Bioinformatics | Scientific Communication | Peer Collaboration