Geng Yu

Indulged in mAb production process development: worked from cell line development, to upstream downstream processes optimization by DOE methods. Cell cell line development: either for high mAB production or cell based assays, enjoying the art of molecular biology tools for cell genetic engineering. Hand-on experience of transcriptomic data mining for identifying targets for mammalian cell productivity improvement. Upstream process development: doing medium analysis to improve medium performance and using DOE study to find optimized cell fed-batch culture conditions. Downstream process development: extensive hands-on experience using AKTA system for affinity, AEX, and CEX columns, virus filtration, Ultrafiltration/diafiltration, and DOE for key purification steps optimization. Able to work within a team and manage project that meet or exceed timeline requirement. Trained and worked with over 15 students to accomplish and design projects for 5 of my publications. Skillsets: • Molecular biology: genomic DNA isolation, quantitative PCR, vector design and construction, mutagenesis, electrophoresis, CRISPR-Cas9 based target selection and implementation • Protein biochemistry: enzyme assays, immunoprecipitation, ELISA, Western blot analysis, SDS page separation, affinity, AEX, CEX, HIC and filtration based purification • Mammalian cell culture, transfection and large-scale cell clone/pool selection experience, extensive large-scale algae culture experience • Analytical chemistry (natural products): GC-MS (for volatile chemicals), HPLC (for amino acids and mAB aggregates), Nova Flex, Vicell, Cedex, BGA and endotoxin detection