Fujun Zhang

Preclinical Drug Discovery and Development  Target driven in vitro biology assays, inculding Kianse, Enzyme, Ion Channel, GPCR, and Nuclear Receptro etc.  Induced Proximity, PROTAC, and RIPTAC platforms  Drug Resistant Cell Lines and Cell Panel platforms  ADC Dual Payload, Novel Payload, and XDC related assay platfroms  Safety Panel and Cardiac Safety evaluation  In vitro ADME, DMPK and in vivo Oncology, Immunology, and CNS Pharmacology etc.

Worked as Scientific Director  Research and Development for company's Cell Sorting, Antibody, and related Cell & Gene Therapy products  Apply and develop new experimental capabilities related to in vitro ADME, Immunology, and Cell Sorting assays  Project management and team management for presenting experimental reports, summarizing data, and writing protocols of the research products  Perform routine maintenance and management of laboratory  Conduct day-to-day problem solving, work prioritization, and co-ordination across the team and clients  Experiment skills including PBMC or Immune cell sorting assays, human primary hepatocyte assays, qPCR, FACS, LC-MS, and enzymatic assays etc.

Worked as Director in Biology department in Hansoh Pharma. Successfully developed a new drug which has been launched or approved in Oncology disease field.  Management of Biology department and projects focused on Oncology, Immunology, Metabolic, NASH, Virology, and CNS diseases  Responsible for project investigation, initiation and management  Responsible for data analysis, project summary & report, IND application for the Preclinical Candidates  Successfully accomplished more than 15 PCC programmes in Oncology, Immunology, Metabolic, NASH, Virology, and CNS fields  Accomplished IND application for more than 10 PCCs and got clinical trial approval in CFDA  Drug screening by in vitro kinase, binding (HTRF), cellular assays and in vivo xenograft assays for oncology drug targets  Drug screening by in vitro binding and cellular function assays for Immuno-oncology drug targets  In vitro kinase, cellular assays, and in vivo assays for drug screening of NASH or liver diseases  In vitro and in vivo assays for drug screening of diabetes or metabolic diseases  In vitro and vivo drug screening for the anti-schizophrenia and depression candidates in CNS fields  In vitro activity screening for large molecules or biologics (antibody drug conjugates or ADC etc.), including FACS & ELISA binding, antibody internalization, and cell killing assays etc.

Worked as Project Leader in biology department in WuXi AppTec, in charge of projects from key clients.  Responsible for the project management, data analysis, work summary & report, and communication with the clients  Target validation research using gene knock down and shRNA technology in oncology field  Stable cell line (knock down) establishment using Lentivirus system  In vitro biochemical and cellular assays for screening small molecular compounds in integrated service project  Cell-based functional assays for screening large molecules (antibodies) in biologics  Experiment skills including siRNA or shRNA design, shRNA construction with Lentivirus vector, virus packaging, target cell line infection, gene knock down stable cell line establishment, Western Blot, HTRF, cell-based ELISA assay, cytotoxicity/viability assay, cell-based apoptosis assay, Ab Hunter RTK assay, growth factor dependent proliferation assay etc.

Worked as Project Leader in biology department in ChemPartner, in charge of projects from key clients.  Responsible for the project management, data analysis, work summary & report, and communication with the clients  Platform Establishment for anticancer drug screening and high throughput screening on compounds  Structure-activity relationship researches on anticancer target and its inhibitors  PK/PD study on the preclinical candidates  In vitro or vivo biology and pharmacology researches on potential anticancer target  Combo or combination study on the candidate inhibitors with other anticancer agents  Gatekeeper and analog sensitive kinase allele (ASKA) researches for POC  Transgene Cell-based kinase selectivity assay  In vitro or vivo pharmacology researches on anti-inflammation target  Drug screening on nuclear receptor (NR) targets  HTS campaign for different projects and compound libraries  Experiment skills including Western Blot, IMAP (Fluorescence Polarization), Meso Scale Technology, LanthaScreen (TR-FRET), Biotinylation, 3D Anchorage-independent Growth Assay, Cell Proliferation/cytotoxic Assay (Cell-titer Glo etc.), Cell-based ELISA, Real Time PCR, Kinase Assay (HTRF, IMAP, Lantha, ELISA etc.), Cell Migration or Invasion Assay, Angiogenesis Assay, Thermo Shift Assay, Cascade Assay, PK/PD Study, In Vivo Xenograft Efficacy Assay, in vivo zymosan inflammation model etc.

Worked as a Manager in biology department in Pharmalegacy Inc.. The research work was focused on in vitro biology and molecular pharmacology research.  Molecular pharmacology, in vitro or ex vivo biology researches on cancer  Molecular pharmacology, in vitro or ex vivo biology researches on immunology  The techniques involve Cell growth/Cytotoxicity Assays (SRB, MTT etc.), Western blot, ELISA, Real-time PCR, Immunohistochemistry, Kinase assays (ELISA), Cell Migration/invasion Assay, Fluorescence Dyeing, and Angiogenesis Assays etc.  GLP training and SOP writing of experiments and equipment

Worked as a Senior Research Scientist in biology department in Ascepion Pharmaceuticals Inc.. The research work focused on the screening of tyrosine kinase inhibitors and anti-angiogenesis agents.  Protein expression and purification of RTK membrane protein in insect cells  Tyrosine kinase assays  Explant aorta ring assay  Isolation of HUVEC and migration assay

Worked as an Associate Director of pharmacology department and editor-in-chief of a company magazine in Zhongqi Pharmaceutical Technology Co., LTD. The research work was focused on preclinical drug discovery.  Initiate new projects for drug discovery  Safety evaluation of new drugs and the experiments supervision  Collecting and sorting out the pharmacological and toxicological files of new drugs to be registered  Drug discovery targeting enzymes for treating diabetes mellitus  Screening anticancer compounds through in vitro cell assays  Screening active ingredient from Chinese traditional medicine by in vivo cancer models  Screening active ingredient from Chinese traditional medicine by in vivo diabetes models  Serum pharmacology researches using big animals