Eva G-ramallo

Gene essentiality in Mycoplasma Pneumoniae, designing a defined serum-free medium and engineering bacteria to deliver therapeutic agents or to present antigens for vaccination of humans and animals.

-cloning, transformation/transfection in bacterial, yeast and mammalian cells -RNA and protein expression -gene reporter activity assays -cell culture (bacterial, yeast, cell lines and primary mammalian cultures) -Lab manteinance and Safety Coordinator

-Handling and maintaining of mammalian cellular culture lines and responsible for cellular culture unit -Lab maintenance (stocks, laboratory orders and wiki implementation) -DNA/RNA extraction from cellular culture and primate tissues -Synthetic genetic devices construction -Transformation/Transfection in bacterial/mammals cells (plasmid DNA/microRNA) -Gene reporter activity assays (RFP/GFP and luciferase) -Sequence analysis (BLAST, Multialin, ClustalW2)

-Maintenance and registry of serum/plasma/blood bank from acute stroke patients -Qualitative/quantitative study of oxidative stress biomarkers at the protein level (Western blot, immunoprecipitation, EIA) -Caracterization/quantification of plasma microparticles (flow cytometry, ELISA).

-Chemokine expression in inflammatory mouse model (RT-PCR, ELISA, animal handling, culture from tissue explants) -Assays in cellular culture, blood isolated cells or tissues and in mouse/rat in order to assess pharmacological activities of in-house synthetized compounds (enzymatic activity, MTT, ELISA, binding).

-Result validation of clinical analysis

-Activity and gene expression of cyclooxygenase and vasoactive agent effects in hepatic stellate cells (isolation of hepatic stellate cells from hepatic resections, primary cellular culture and cell culture of stablized lines, RIA, RNA isolation, RT-PCR, Northern blot and Western blot)