Eric Cheah

• Designed and executed complex flow-based assays (including PrimeFlow) to support critical pharmacology studies towards DC nomination for Generation Bio’s first-in-class LNP platform delivering siRNA to T-Cells. • Developed, optimized and implemented novel assays evaluating TA biodistribution across T-cell lineages and its effect on T-cell activation in humanized murine models of autoimmunity (e.g. GvHD). • Analyzed and communicated key data upwards and cross-functionally on tight timelines to support company milestones.

• Developed novel ex vivo murine tissue analysis workflows, including complex tissue dissociation and primary cell isolation and culture, and cross-trained other teams on these new workflows. • Developed and optimized tissue dissociation techniques and associated flow cytometry panels to identify and characterize tissue residency of immune cell populations in ex vivo murine tissues (liver, small and large intestines, skin). • Led in vivo pharmacology studies to evaluate T-cell receptor and tool ligand association and assess biodistribution of cell-targeted lipid nanoparticles (ctLNPs) in transgenic mice. • Designed and executed in vitro experiments using primary murine cells to assess tool siRNA specificity. • Provided cross-functional support by screening antibodies as part of novel target validation efforts. • Shared new data, initiated discussions and solicited feedback around data interpretation in bi-weekly Immunology meetings.

• Designed immunophenotyping panels for the characterization of hematopoietic stem and progenitor cells (HSPCs) from cord blood, bone marrow and mobilized peripheral blood in vitro as part of Generation Bio’s HSC program for sickle cell disease and beta-thalassemia. • Independently designed and executed in vitro assays to assess delivery of ctLNPs to HSPCs and evaluate expression of LNP cargo. • Led in vitro experiments to identify and validate primary HSPCs and utilized them to perform target selection and optimization of uptake and expression assay conditions. • Initiated and implemented colony-forming unit (CFU) assay to evaluate functional activity of HSPCs. • Provided Molecular Biology team with flow cytometry support in in vitro PoC gene editing studies. • Presented key experimental updates and drove data discussion on follow-up experiments in cross-functional team meetings.

• Coordinated and performed the routine isolation of primary cells from murine livers to meet the research needs of the Preclinical Research team. • Assisted in the development and optimization of cell- and plate-based assays to evaluate primary cell or tissue samples for expression and functional activity. • Designed and executed cell-based ex vivo and in vitro experiments for the screening and characterization of potential gene therapy drug candidates

• Drove the development, optimization, execution and analysis of time course and washout experiments to inform understanding of a novel antibody drug conjugate (ADC) in Phase I/II clinical development for hematopoietic stem cell transplant in hematology/oncology and acute myeloid leukemia (AML). • Led experiments investigating the detailed internalization kinetics of MGTA-117, a novel, clinical-stage ADC. • Scientific lead for external collaboration evaluating patient AML samples. Drove laboratory experiments, data analysis, and meetings with collaborators. • Established processes for receipt of patient samples from ongoing clinical trials, including clear record keeping, storage, and processing in collaboration with quality team. • Presented key highlights of studies to research team, including CSO, and Heads of Medical, Clinical Pharmacology, Clinical Development, Preclinical Development and Program Management.

• Performed immunophenotyping and immune cell functional assay (T-cell expansion assay) on non-human primate (NHP) peripheral blood for comprehensive characterization of immune cells for pre-IND data package. • Optimized detailed assay procedures to ensure robustness for clinical use, including flow-based receptor occupancy assay. • Provided critical support for a collaboration with an academic collaborator by processing patient samples, as well as generating and presenting data. • Executed cellular biology antibody and antibody drug conjugate assays (e.g., binding assay, killing assay) efficiently with readouts using flow cytometry/ CellTiter-Glo.

• Supported the execution of in vivo pharmacology studies to assess early drug candidates by performing blood collection, necropsy and tissue collection. • Worked on translational projects to characterize the hematopoietic stem cell engraftment potential of cells mobilized with a novel, Phase II clinical mobilization regimen (MGTA-145) • Assisted in the execution of pre-clinical, IND-enabling studies for novel conditioning ADC in both a PDX AML model and an allogeneic transplant model. • Independently acquired and processed samples from murine studies (peripheral blood, bone marrow, spleen, thymus), including tissue processing and analysis by flow cytometry. • Managed multiple cohorts of immunocompromised and immunocompetent mice. • In Vivo Skills: Dosing (PO, IP, IV, SQ), blood collection (submandibular, retro-orbital, cardiac stick, tail vein venipuncture), tumor measurements, irradiation, tail tattooing, anesthesia, euthanasia

Project: Determine the role of CEACAM1 in T-cell regulation in murine models of inflammatory bowel disease (IBD), pancreatic cancer and melanoma • Dissociated patient tumor samples to obtain tumor infiltrating lymphocytes and isolated peripheral blood mononuclear cells from patient blood, subsequently performed phenotypic analysis and in vitro functional assays with the acquired cells. • Worked extensively with animal models (DSS/ T-cell transfer colitis models and cancer models), organ harvesting and tissue processing for further analysis. • Cultured multiple human and mouse immortalized cell lines. • Carried out plate-based assays, such as ELISAs and cross-blocking assays. • Generated gene expression data via qPCR and western blot. • Performed transformation, maxi/miniprep for plasmid isolation, transfection and luciferase assay. • Supported mouse colony maintenance efforts and performed genotyping by agarose gel electrophoresis. • Washed and autoclaved glassware, as well as ordered and stocked lab supplies as needed.

• Authored and reviewed documentation to ensure compliance with FDA regulations. • Performed various tests to fine-tune an automated cell culture system, such as tubing tensile strength, pH sensor function, and gas flow rate and maintenance tests. • Programmed test equipment, such as INSTRON testing system and PID controllers. • Produced and maintained equipment logs for new laboratory equipment.

• Performed immunohistochemistry and immunofluorescence stainings on rodent brain tumor sections and performed analyses including confocal microscopy. • Calculated the mitotic index of rodent brain stainings along with core-shell ratio. • Computed human brain tumor (glioblastomas and astrocytomas) tractography and tumor volume. • Studied T2 flair images of brain tumors.

Combined program: Graduate Certificate in Nanomedicine

Transferred to Northeastern University.