Emily Pool
Development Scientist @ Immunis, Inc.
-
United States
Biotechnology
Bacterial Cell Culture, Enzyme Kinetics, Molecular Cloning, Protein Purification, Mammalian Cell Culture, Biophysics, Cell Signaling, Microscopy
Experience

Graduate Student Researcher
Professors Jin Zhang and Susan Taylor groups • Characterized protein kinase A RIβ phase separation in mammalian cells and in minimal reconstitution systems • Expressed genetically encoded biosensors in mammalian cells to quantify the impact of PKA RIβ liquid-liquid phase separation on the spatiotemporal regulation of protein kinase A signaling • Evaluated and developed a model of how neurological disease mutations affect RIβ phase separation and cell signaling • Utilized fluorescence recovery after photobleaching (FRAP) to quantify the apparent diffusion and exchange of RIα in wild-type and mutant phase separated system

Teaching Assistant
UC San Diego Department of Chemistry and Biochemistry • Course: General Chemistry Laboratory (CHEM 7L), General Chemistry (CHEM 6B), Organic Chemistry Laboratory (CHEM 43A) • Prepared weekly discussions to explain chemical concepts, gave weekly office hours, graded examinations and laboratory notebooks

Undergraduate Laboratory Assistant
• Courses: Advanced Biochemistry Laboratory, Biochemistry Laboratory, General Chemistry Laboratory • Topics: bacterial protein expression, enzyme kinetics, acid/base chemistry, spectroscopy • Prepared laboratory materials and calibrated equipment, answered student questions

Undergraduate Student Researcher
Indianapolis, Indiana, United States
Professors Geoff Hoops' and Jeremy Johnson's groups • Evaluated the substrate specificity of novel esterases from Mycobacterium tuberculosis using a library of latent fluorophores • Cloned and purified bacterially expressed proteins
Emily Pool's Contact Information
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