Eleni Kisty

Research Advisor: Eranthie Weerapana • Mastered diverse quantitative chemoproteomic techniques including: IsoTOP-ABPP, SILAC, ReDiMe, OxICAT, organelle isolation, enzymatic proximity labeling (TurboID/APEX2), OxICAT, CETSA, off-line fractionation, immunofluorescent confocal microscopy, small molecule and fragment-based target identification • Experienced user of Thermo Orbitrap LTQ, Orbitrap Discovery, and Exploris mass spectrometers and Easy nLC and Agilent LC systems • Obtained extensive experience with mammalian cell culture and engineering, lentiviral and transient transfections, immunoprecipitation, cloning, site-directed mutagenesis, recombinant protein expression, click chemistry, phenotypic screens, enzymatic assays and organic synthesis • Developed innovative quantitative mass spectrometry platforms combining organelle isolation, proximity labeling and quantitative proteomics for studying intracellular oxidative stress response on cysteine oxidation • Identified phenotypic impact, protein targets and site of labeling for numerous covalent/non-covalent inhibitors including a library screen of 49 drug-like small molecules in MCF10CA1a breast cancer cell line • Proficient in Proteome Discoverer and in-house mass spectrometry analysis programs

Advisor: Lindsay Comstock Honor's Thesis: Synthesis of DOT1L Substrate-Profiling Probe • Engineered synthetic routes to S-adenosyl-L-methionine cofactor analogues to target DOT1L and DOT1L substrates while problem solved and reinvented schemes to overcome challenges • Synthesized, purified, and characterized small molecule intermediates via NMR, HPLC, GC-MS

Thesis: Chemoproteomic Methods to Evaluate Cysteine Oxidation in the Mitochondria

Concentration: Biochemistry Minor: Biology