David Walgraffe
Scientist Expert @ Imcyse SA
About
Since the beginning of my studies, my motivation has always been to participate in projects developing innovative scientific solutions for the benefit of people's well-being. The various positions I have held so far in preclinical R&D have allowed me to explore different environments, meet talented people, and acquire valuable technical and interpersonal skills. I recently took on a new challenge by pursuing advanced training in Data Science. Throughout my journey, my goal has always been to thrive in my role by leveraging my experience while continuing to learn.
Belgium
Liège
Biotechnology
Project Management, Team Management, Compliance Documentation, Multiple Project Coordination, Priority Management, Cross-team Collaboration, Data Presentation, Multiple Sclerosis, Development of animal models, Immunoassays, ELISA, ELISPOT, Flow Cytometry, Mammalian Cell Culture, Immunology, Chromatography, Protein Expression, Molecular Biology
Experience
Scientist Expert
Imcyse SA
Liège, Walloon Region, Belgium
Team Leader
Imcyse SA
Liège, Walloon Region, Belgium
Project Preclinical Leader
Imcyse SA
Liège Area, Belgium
Project Leader
Imcyse SA
Liège Area, Belgium
Scientific Officer
Imcyse SA
Liège
Scientific Officer in the laboratory of Experimental Allergology (Dr A. Jacquet and Dr E. Adam)
Université libre de Bruxelles
Development of recombinant allergens for specific immunotherapies PUBLICATIONS 1. Bouaziz A, Walgraffe D, Bouillot C, Herman J, Foguenne J, Gothot A, Louis R, Hentges F, Jacquet A, Mailleux AC, Chevigné A, Galleni M, Adam E, Dumez ME. (2015) Development of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy. Clin Exp Allergy. 45(4), 823-34. 2. Walgraffe, D., Mattéotti, C., el Bakkourry, M., Garcia, L., Marchand, C., Bullens D., Vandenbranden, M. & Jacquet, A. (2009) A hypoallergenic variant of Der p 1 as candidate for mite allergy vaccines. J. Allergy Clin. Immunol. 123(5), 1150-6.
PhD Thesis performed in the laboratory of Molecular Parasitology (Prof E. Pays and Dr L. Vanhamme)
Université libre de Bruxelles
Study of the mechanisms controlling the expression of VSG genes in Trypanosoma brucei. PUBLICATIONS 1. Uzureau, P., Daniels, J. P., Walgraffe, D., Wickstead, B., Pays, E., Gull, K. & Vanhamme, L. (2008) Identification and characterization of two trypanosome TFIIS proteins exhibiting particular domain architectures and differential nuclear localizations. Mol. Microbiol. 69(5), 1121-36 (The two first authors contributed equally to this work). 2. Lecordier, L., Devaux, S., Uzureau, P., Dierick, J.-F., Walgraffe, D., Poelvoorde, P., Pays, E. & Vanhamme, L. (2007) Characterization of a TFIIH homologue from Trypanosoma brucei. Mol. Microbiol. 64(5), 1164-81. 3. Devaux, S., Lecordier, L., Uzureau, P., Walgraffe, D., Dierick, J.-F., Poelvoorde, P., Pays, E. & Vanhamme, L. (2006) Characterization of RNA polymerase II subunits of Trypanosoma brucei. Mol. Biochem. Parasitol. 148(1), 60-8. 4. Lecordier, L., Walgraffe, D., Devaux, S., Poelvoorde, P., Pays, E. & Vanhamme, L. (2005) Trypanosoma brucei RNA interference in the mammalian host. Mol. Biochem. Parasitol. 140, 127-31 (The two first authors contributed equally to this work). 5. Walgraffe, D., Devaux, S., Lecordier, L., Dierick, J.-F., Dieu, M., Van Den Abbeele, J., Pays, E. & Vanhamme, L. (2005) Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei. Mol. Biochem. Parasitol. 139, 249-60.
Education
Université libre de Bruxelles
Biology/Biological Sciences, General
Molecular Parasitology Laboratory Professor E. Pays and Dr L. Vanhamme Study of the mechanisms controlling the expression of VSG genes in Trypanosoma brucei.
Université de Namur
Applied Biology
Project performed in the laboratory of Microbiology-Immunology Professor J.-J. Letesson and Dr P. Lestrate Training period (5 months) performed in the University of Manchester (UK) Professor K. Gull and Dr K. Ersfeld
Université de Namur
Biological Sciences
Graduate thesis performed in the laboratory of Microbiology-Immunology Professor J.-J. Letesson, Dr P. Mertens and Dr X. De Bolle PUBLICATION 1. Mertens, P., Walgraffe, D., Laurent, T., Deschrevel, N., Letesson, J.-J. & De Bolle, X. (2001) Selection of phage-displayed peptides recognised by monoclonal antibodies directed against the lipopolysaccharide of Brucella. Int. Rev. Immunol. 20, 181-99.
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