Dr. Yugantak Raj Gupta

I'm a translational molecular biologist and genome editing scientist with 6+ years of research experience spanning academic science and biotech R&D.My current focus is CRISPR/Cas9-based gene therapy. At Helex, I work on developing dual guide RNA-mediated excision strategies to remove mutations driving chronic kidney disease — validating editing outcomes across 5 cell lines, including HEK293, WT9-7, TKPTS, VERO, and mIMCD-3.My technical toolkit covers the full genome editing workflow:→ CRISPR/Cas9 design and dual gRNA deletion strategies→ Lipofectamine-mediated transfection optimization→ PCR, qPCR, Sanger sequencing, Western blotting, immunofluorescence→ Flow cytometry and NGS-based editing validation→ Assay development, troubleshooting, and translational pipeline supportBefore transitioning into gene therapy, I earned my PhD from the University of Hyderabad, where I pioneered the first-ever isolation of follicular cells in Cyprinus carpio and demonstrated the role of thoc3 and Pentraxin in steroidogenesis during oogenesis. My doctoral work resulted in 2 first-author publications — one recognized as the journal's most cited manuscript (2019–2020) in General and Comparative Endocrinology.I've also qualified the CSIR-UGC National Eligibility Test (NET) — a nationally competitive examination for research and academic eligibility in India.Core expertise:CRISPR/Cas9 genome editing | Mammalian cell culture | Functional genomics | Gene expression analysis | Assay development & optimization | Flow cytometry | PCR & ddPCR | Translational R&D | Scientific documentation