Dr. Nikita Singh

● Independently designed, planned, monitored, and reported nonclinical in vivo (rodents, rabbits and minipig animal model) and, to a lesser extent, in vitro studies in the field of acute radiation syndrome and radionuclide research. ● Proactively managed projects, ensuring strict adherence to quality standards, timelines, and budget targets, resulting in consistently successful outcomes. ● Contributing to the creation of development plans, study protocols, reports, supporting documents, regulatory submissions, and ethical applications ● Provided comprehensive supervision and guidance to study centers at an international level, both remotely and in-person, effectively driving their performance and ensuring the highest standards of execution. ● Aligned the non-clinical program with regulatory strategy and incorporating project-relevant nonclinical data into regulatory documents such as IB, Pre-IND, and IND briefing book for FDA and EMA interactions.

Developed extensive experience in range of molecular, immunological and cellular techniques. ● Molecular biology: Cloning, PCR, RT-qPCR, Transformation, cDNA synthesis, DNA/RNA isolation, CRISPR/Cas9 technique. ● Cell culture: Primary cells, mammalian and murine cell lines, co-cultures. ● Protein Isolation and quantification (Co-IP, SDS-PAGE and western blot). ● Immunology: Flow cytometery, ELISA, Immuno-electrophoresis, cell cycle and proliferation assay, Apoptosis assay, Immunohostochemistry (IHC). ● In-vivo: Mouse model, FELASA B certification, i.p. and i.v. injections. ● Software: MS office, Inkscape, Flowjo, Prism, Endnote. Data interpretation and visualization. Concise communication skills with experience in meticulous scientific data presentation and database search. ● Gained skills in generating robust and reliable data, ensuring studies and results were in compliance with the respective protocol.

Functional characterisation of C3 subunit in Chlamydomonas reinhardtii: ● Molecular cloning (PCR, qPCR, Restriction analysis, Ligation, Transformation, DNA/RNA isolation) ● Chloroplast isolation ● Protein isolation and quantification (Co-IP and western blot) ● Basic bioinformatics ● Microbiology techniques ● Immunology techniques (ELISA, Immuno-electrophoresis)

Handling social media,content writing, editing and managing public relations.

Worked on promotions in different market. -Responsible for sales monitoring/tracking and price refreshes directly on the amazon website. -Helped in building a tool which reduced the number of team strength. -Responsible to monitor and ensure the accuracy of the pricing analytics team through audits and process improvement activities -Perform other duties as assigned, including ad-hoc analysis on methods to optimize price through market and pricing strategies. - In coordination with project managers, prepare projections of revenue and expenses for projects based on numerous variables and data assumptions. -Take initiative in identifying and resolving issues around pricing and how to effectively improve the process to be more competitive in the market. - Monitor, analyze, and report on weekly, monthly and yearly basis for Price Realization, selection and availability in market versus various competitor.