Dr. ATUL PATIL

Technical Skills & Professional Expertise Total Experience: 16+ Years 🔬 Molecular Biology & Biotechnology DNA and RNA isolation, purification, quantification, and dilution PCR master mix preparation, cDNA synthesis, RT-PCR, qPCR, and primer design Restriction digestion, standard cloning, bacterial transformation, and plasmid handling Recombinant protein expression in E. coli and protein purification techniques Analytical techniques: SDS-PAGE, Agarose Gel Electrophoresis (AGE), Western Blotting, Zymography, and ELISA 🧬 Bioinformatics & Sequence Analysis DNA sequencing data analysis using BLAST Motif identification in DNA and protein sequences Nucleotide sequence submission to GenBank Phylogenetic and molecular analysis using MEGA 6 and MEGA 11 📊 Data Analysis & Computational Skills Python programming for data analysis and AI-oriented workflows Data manipulation and analysis using pandas Visualization using matplotlib and seaborn Correlation matrix analysis and heatmap generation Box plot visualization and exploratory data analysis Multivariate analysis including Principal Component Analysis (PCA) using scikit-learn 🧪 Laboratory Operations & Quality Systems Development and implementation of Standard Operating Procedures (SOPs) Preparation of buffers, reagents, and culture media for molecular biology workflows Maintenance, calibration, and troubleshooting of laboratory equipment Accurate documentation and record-keeping (electronic systems, logbooks, and lab notebooks) 📝 Communication, Compliance & Professional Skills Preparation of research manuscripts following national and international journal guidelines Strong ability to work independently with minimal supervision Continuous learning through literature review and tracking emerging scientific trends Ensuring compliance with laboratory quality systems and regulatory requirements Hands-on experience with SAP (QA32), MS Office, and NABL 17025 quality standards