diego segovia

* ●Expression of shRNA Using a Newcastle Disease Virus Vector * Design shRNA directed against the green fluorescent protein (GFP) * Clone shRNA-miR cassette against GFP (shRNA-miR-GFP) into the genome of a transfer plasmid, then an NDV strain (F3aa) * Assess expression of shRNA-miR-GFP ●Retrospective Case Study on Avian disease in small backyard flock operations in Ontario * Examining pathology reports from testing performed at the Animal Health Laboratory (AHL) * Characterizing and identifying trends in avian disease in small flock operations ● Clinico-Pathological Characterization Of Recently Isolated Newcastle Disease Virus Strains in Quail * To describe the clinical signs (Coturnix japonica) caused by virulent NDV strains in Japanese quail using histology and immunohistochemistry Other Project of industry and academic relevance were also performed.

Mass Spectrometry - MALDI-TOF Verification Lead, R/D/Validation Scientist, Lab supervisor Microbiological Research and Instrument Validation: ● Assess software and instrument performance with live specimens ● Conduct experiments for research and development, and assess instrument capabilities ● Prepare user and company documents for usage of the instrument Verification: ● Verifying product robustness and software usability ● Creation, maintenance and execution of product test suites ● Aid to establish and define high level requirements Biosafety: ● Work to equip and maintain facility laboratories with equipment appropriate for work ● Train and demonstrate proper microbiological technique and research experiment design to lab staff and assistants Software: ● Tracking verification progress, project issues, risks and requirements ● Using provided test tools and agile processes to assist Developers and for testing ● Manage the tools, frameworks and verification environments

●Assisted nurses and secretaries and doctors ●Interacted with patients & staff

* ● Create, oversee and maintain a budget of ~$100,000 * ● Supervise and authorize all spending and income with the council * ● In charge of issuing cheques * ● Main contact for petitions, delegations, and requests, ~ $10,000 in funding to award to student and administration run initiatives * ● Finance Committee Chair * ● Financial advisor and supervisor to College of Biological Science Clubs

* ● Fungi preparation, inoculum, culturing, and analysis * ● Media preparation * ● Sample set-up, and evaluation * ● Wheat breeding for resistance to the filamentous fungi Fusarium

* ● Supported the research projects of faculty, postdoctoral, graduate and undergraduates * ● Facility maintenance, distribution of biological control; environmental programming, maintenance of the greenhouse and plant growth chambers * ● Maintenance and caretaking of plant collection and plant research subjects

* ● Received and prepared specimens and samples * ● Cultured samples using traditional and technological diagnostic bacteriology assays * ● Prepared and handled laboratory paperwork, organizational and administrative assistance * ● Data entry into Safire software and Windows software on testing results and antibiotic resistance testing

Activities and Societies: Ontario Students Against Impaired Driving, Jazz Band, Concert Band, Development and Peace, Debate Team

Activities and Societies: Interhall Council, Laboratory Services, College of Biological Sciences Student Council, Judical Commitee, Guelph Residence Recognition Program