David Piñeiro, Ph.D.

CMC responsible at aptaTargets @ aptaTargets

Country

Spain

City

Madrid

Industry

Biotechnology

Skill

Cell culture: bacteria, mammalian cell lines, inducible stable cell lines, RNA-protein interactions: EMSA, RBP-capture, RIP assays, Experience guiding and supervising small groups (Technician, PhD student, PhD), Gene regulation expression: siRNA, shRNA, Flow Cytometry, Microsoft Office, Immunoprecipitation techniques: IP, co-IP, RIP, RNA based assays: in vitro transcription, in vitro translation, EMSA, Cell based assays: ROS analysis, cell death, viability and citotoxicity assays, Biochemistry: ELISA, ELONA, Immunofluorescence, Protein Engineering, protein expression and protein purification, Protein-protein interaction: co-IP, FRED, BRED, TAP assays, ImageJ, Adobe Photosho, GraphPad Prism, Polysome profile, Critical and creative mind to generate, plan and execute projects

Experience

CMC responsible at aptaTargets

aptaTargets

2019-5 - Present · 7 yrs 5 mos

Project Manager

aptaTargets

2018-9 - Present · 8 yrs 1 mo

Madrid Area, Spain

Investigator Scientist

MRC Toxicology Unit

2013-1 - 2018-2 · 5 yrs 2 mos

Leicester, United Kingdom

Identification and characterisation of new RNA binding proteins after nucleolar stress by RBP-capture and mass spectrometry Project management and staff mentoring Scientific writing reports and presentations New techniques development

Postdoctoral position (Sara Borrell Grant)

CBMSO-CNB

2008-12 - 2012-12 · 4 yrs 1 mo

Madrid, Spain

Identification and characterization of RNA binding proteins regulating IRES- dependent translation during RNA virus infection Team leading, project management and experimental design International collaboration and internship (2013-2014 at MRC) In charge of orders, SOP’s, equipment and data presentation

Ph.D. Student

Hospital Ramón y Cajal

2003-1 - 2007-7 · 4 yrs 7 mos

Madrid, Spain

Characterization of translational regulation in apoptosis induced by taxol International collaborations and meetings participation Data analysis and articles writing.

Visiting Ph.D. Student

CHEO Research Institute

2006-5 - 2006-8 · 4 mos

Ottawa, Canada

Successful generation of eIF4E mutants at the S209 residue to achieve mimic phosphorylation or dephosphorylation states.

Summer Research Student

Universidad de Alcalá

2002-6 - 2002-9 · 4 mos

Alcala de Henares, Spain

In this period, I was involved in a project which goal was to select DNA aptamers against Leishmania infantum proteins. The selection methodology is known as systematic evolution of ligands by exponential enrichment (SELEX).