Daniela Pontieri
Senior Scientist in Histology and Image Analysis @ Adcendo
About
I am a PhD level molecular and cell biologist with bioinformatic skills and passion for innovative research. I have 12 years of international inter-disciplinary work experience from both academic and biotech environments. I have mainly focused on investigation of gene and protein expression in animal and in vitro models. I have profound hands-on experience with standard and specialized molecular techniques and biochemical approaches, as well as with bioinformatic RNA- and whole genome sequencing data analyses. I am particularly trained in molecular cloning, gene and protein expression analyses, in sterile techniques for work with cell cultures, specifically with mammalian, primary and insect cell lines, and in diverse microscopy techniques. In addition, I have solid experience in recombinant protein expression, isolation and purification. Moreover, I have experience with running administrative and operational tasks for a start-up biotech company: from procurement and meetings organization, to onboarding of new colleagues and webpage design. As a naturally organized and structured person, I have ability to hit short and focused deadlines and I thrive in an innovative multinational collaborative environment. I am an effective communicator with meticulous documentation and results presentation. As a colleague, I am very collaborative and I value inputs from all the colleagues, as I like to participate daily in results and data discussions. I am a person with positive attitude, natural curiosity and willingness to help at any time. Main competencies: R&D Research Scientist | Drug Discovery | In Vitro Models | Assay Development | Molecular and Cell Biologist | Cell Cultures | Mammalian, Primary and Insect Cell Lines | Molecular Cloning | Gene and Protein Expression Analyses | Genome Editing | Protein Expression and Purification | Microscopy and Image Analysis | NGS and WGS Data Analyses | Scientific Administration and Operations Coordination
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Denmark
Biotechnology
TUNEL assay, Data-driven Decision Making, Whole Genome Sequencing data analysis, ELISA, Benchling, circular RNA, Transfection, Primary Cells, Cell Biology, Time Management, Skilled Multi-tasker, Oral Communication, Microsoft Office, Management, Logistics Management, Executive Administrative Assistance, Communication Training, Communication, Calendaring, Scientific Background
Experience

Senior Scientist in Cell Biology
Aloop Therapeutics
Copenhagen, Capital Region, Denmark
I played a role in the development of innovative circular RNA therapeutics for treatment of metabolic diseases. I obtained skills in work with in vitro models, protein quantitation and immunoassay, but also in establishing company´s laboratory set-ups and in Benchling implementation. I successfully set up and characterized indication-specific models, evaluated endogenous expression of indication-specific protein and expressed this protein from circRNA in patient derived primary fibroblasts.

Research Scientist
Hørsholm, Capital Region, Denmark
I obtained hands-on skills in perfusion fermentation in order to optimize upscaling process of protein production in Drosophila cell line. I also developed transient and stable insect cell lines via transfections. In addition, I independently developed a pipeline for whole genome sequencing data (Oxford Nanopore Technologies) analysis.

Maternity leave
2nd child
Copenhagen, Capital Region, Denmark

Operations Coordinator
Hørsholm, Capital Region, Denmark
I coordinated company´s administrative tasks and provided organizational and communication support in order to secure colleagues´ smooth daily workflow by daily communication with key suppliers, by securing company´s stock of laboratory materials and office supplies, by organizing computer equipment for new employees, by onboarding of new employees, all in coordination with a finance team. I was also responsible for answering and forwarding calls, as well as for webpage updating and design via WordPress.

Research Scientist
I investigated sensory response to the bath applied neurotransmitters (synthetic neuropeptides) via extracellular electrophysiology and expression patterns of visual proteins in box jellyfish via immunohistochemistry.

Maternity Leave
1st child
Copenhagen, Capital Region, Denmark

Postdoctoral Researcher
Cologne, North Rhine-Westphalia, Germany
During my postdoc position I was responsible for RNA-sequencing data analysis of 30 transcriptomes of 4 developmental stages of bug species Oncopeltus fasciatus. I generated list of differentially expressed genes during important developmental events of the embryogenesis.
Daniela Pontieri's Contact Information
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