Christopher C.

• Managed the full sales cycle for Incucyte across the Nordic region, from opportunity generation through contract closure. • Partnered with academic, biotech, and pharmaceutical customers to develop workflow solutions that accelerated research and drug discovery programs. • Drove territory growth through strategic account management, technical presentations, demonstrations, and cross-functional collaboration with applications, service, and support teams. • Delivered the first European sale of the newly launched Incucyte NPI platform.

• Spearheaded the coordination of impactful events and strategic marketing campaigns • Acted as a liaison between laboratories and Proteintech, fostering scientific communication • Authored informative technical blogs focused on molecular biology techniques

My role at the Department of Translational Medicine in Lund University has involved me working across Sweden, UK, US, and Denmark in several different key projects such as: 1) Hypoxia Generated by Avian Embryo Growth Induces the HIF-α Response and Critical Vascularization 2) Cisplatin resistance vis HIF2a and AP1 singalling. 3) Multicellularity, biomineralization and their regulation by circadian CLOCK 4) Targeting ATF3 signalling in response to radiotherapy. Other tasks include: 5) Co-supervision of undergraduate and Ph.D students (3 undergraduate students , 2 Ph.D students) 6) Key contact person for protein based experiments within the Tissue Development and Evolution labs in multiple model organism systems. 7) Superuser of XenX and chemidoc systems 8) Generation of CRISPR Knockouts, Knockins, and CRISPRi in multiple cell lines, targets, and species.

Training in the generation of Single Cell Whole Genome Sequencing datasets.

Main Project: Targeting hypoxia regulated sodium driven bicarbonate transporters (NDBT) to reduce breast cancer metastasis Undertook a research project into the molecular mechanisms of pH regulation, hypoxia, and cancer metastasis. The project involved investigating changes at: epigenetic, transcriptional, protein, and function levels. Fusion proteins were produced to determine binding partners assays and co-localisation. In vitro migration and invasion assays were performed; as well in vivo experimentation to determine the effect upon metastasis. Mechanistic investigations determined that NDBT knockdown or inhibition reduced mTOR activation and HIF-1 stabilization in hypoxia. This culmiated in a loss of EMT induction in knockdown cells. Furthermore, metablomics and metabolism based assays were performed to determine the effect on ATP production, lacatate, ROS levels, and mitochondrial copy number and function. Side project 1: Sound based imaging techniques for cancer biology Side Project 2: Cancer cell evolution and cellular plasticity

Contracted to work for GSK within the High Throughput Expression and Characterization group. Undertook protein analytics within Biopharm discovery to characterize antibodies for multiple projects and feed back results to project teams. This required the bespoke designing of each assay for each project or piece of data required. Tasks included epitope binning, active protein concentration quantification, affinity and kinetics determination, single shot binding screens, LC-Mass spec, and size exclusion chromatography etc. This was primarily performed using Biacore and Octet. Adittionally, MSD-SET assays were also performed.

Contracted to work for by VWR Catalyst as a research technician in Oncology iMED. Required to ensure the smooth operation of the high throughput screening of novel small molecule inhibitors in the search for synergistic effect. This project required the maintenance of 250 cell lines from a plethora of tissue types and correct dosing of 50 different compounds in a 384 plate well format. Automated systems used include Acumen and echo. The data was analysed using genedata, spotfire and other analysis packages

Undertook a variety of tasks ensuring the smooth running of the Whole Cohort SNPs genotyping project (WCSG). This included quality control testing, DNA extraction and preparation, and also process validation. In addition, I also edited standard operating procedures, performed molecular biology assays, and used robotic systems whilst ensuring all data was properly logged in LIMS.

General cleaning and maintenance of working, serving and communal/social areas. In addition I was required to regularly check, maintain, and replenish existing stock.

Masters course encompassing a large array of disciplines aimed at developing a well rounded scientist prepared for further research in areas related to cancer biology. I studied areas from Tummor immunology and physiology to statistics and drug design. Also I studied a variety of laboratory techniques aimed at providing me with the skills required to enter many labs and work effectively. For my thesis I investigated the interactions between a novel anti-apoptotic protein and a HECT family E3 ligase. My investigations required I master western blotting, co-immunoprecipitations, protease protection assays, and transfection techniques in addition to maintaining HEK 293 cells.

Undergraduate degree encompassing multiple areas within the field of life-sciences. I studied areas such as: Genetics, Pathology, and Microbiology. My research project Investigated the effectiveness of different chemical softening agents on morphology and staining intensity on nail speciemens.

Second year of my undergraduate degree studied abroad as an exchange student. I undertook a wide variety of classes aimed at giving me a broadening my understanding of human biology. Whilst a student I covered topics and participated in laboratory experiments covering: genetics, microbiology, histology, and nutrition. In addition, there was discussion of many contentious issues in biology were discussed and rigorous practice of paper critiquing.