Chen Chen

Critical Skill Set • Molecular analysis of protein and RNA: Western blot analysis, ELISA, Luciferase assay, Luminex, PCR and real-time qPCR • Immunostaining (IF and IHC) • PCR and real-time qPCR • Cell culture ♦ Perform micromanipulation technique to isolate and culture PGCs (Primordial germ cells), and MSC (Mesenchymal stem cells) ♦ Isolate and culture HBECs (Primary Human Bronchial Epithelial Cells) ♦ Air-Liquid Interface Culture: HBECs (Primary human bronchial epithelial cells), tracheal epithelial cells ♦ Culture primary mouse lung tumor cells • Managed mouse colony ♦ Genotyping and geneclean ♦ Experience in breeding wild type and transgene/knockout mice • Animal experiments ♦ Perform microdissection of mouse lung tumors ♦ Collect BAL, blood and tissue sample ♦ Perform i.v. (Mouse tail intravenous injection), i.t.(intratracheal instillation),and i.p.(intraperitoneal injection) injection on the mouse ♦ Perform cryosections and paraffin sections of mouse tissues ♦ Extract and analyze protein, RNA, and DNA from the mouse