Charlotte Kelley

• Worked on 20 client projects, several of which were as project lead. Responsibilities included proposal writing, experiment planning and execution, analysis, dissemination of results, and writing monthly expense reports. • Developed high-content screens for clients in 384 and 1536-well format to quantify localization and signal intensity changes in target proteins. • Developed and optimized biochemical and cell-based assays to screen compound libraries, verify targets, and understand downstream signaling pathways of target proteins. • Optimized SPR protocols for ligand immobilization and analyte screens for SAR campaigns and client compound development for 9 projects. • Optimized and performed HTRF assays on cell lysate and mouse tissue samples to inform PK/PD client studies • Managed two direct reports, mentored several others at both the Research Associate through Scientist level

Leveraged expertise in cell and molecular biology and bioengineered matrices through complementary in vivo and in vitro systems to understand how triple negative breast cancer metastasizes despite chemotherapy treatments. Focused on the role proteases play in altering the extracellular tumor microenvironment by digesting the extracellular matrix, other proteases, and cell surface receptors. These same proteases can also perturb the intercellular signaling and cellular organization. I found that depending on the protease, these changes can be both pro- or anti-tumorigenic. Other roles include: Head of Lab Safety Undergraduate Mentor Organizer for the Summer Program "Engineering Investigations"

Through a collaboration between the Global Resilience Institute (Northeastern University) and Lincoln Labs (MIT), worked on a cross functional team to collect data, conduct interviews, and assess the impacts of the COVID-19 pandemic on 18 communities across New England with the goal of writing a report containing findings and recommendations for the Federal Emergency Management Agency (FEMA), to allow states to apply for federal funds to ensure a resilient economic recovery.

Part of a team of three graduate students and post-docs that managed all aspects of the Biology Department LSM710 Zeiss scanning confocal microscope training, use, and maintenance. Helped troubleshoot problems and coordinate with Zeiss representatives, as service was needed.

I study cells communicate intra- and intercellularly via biochemical and mechanical signals to coordinate contractility across tissues. To address these questions I have: I. Generated and screened a RNAi library of over 150 genes to find novel regulators of contractility which led to the identification and characterization of a myosin light chain kinase II. Utilized imaging, molecular, and genetic techniques to map a spatial protein oxidation gradient across the C. elegans spermathecal tissue and found a functional role for this in regulating Rho activity through oxidation of a specific cysteine III. Used extensive live imaging techniques such as time-lapse 3D microscopy and fluorescence recovery after photobleaching (FRAP), immunofluorescence of fixed samples, and generation of transgenic animals to uncover a role for filamin, an actin crosslinker, in not only regulating actin network organization, but also in organizing the localization and distribution of proteins and organelles IV. Established and managed collaborative projects within and between labs to present oral and poster presentations, and to publish several manuscripts Other duties include: Lab Manager Acted as primary liaison between sales representatives and the lab, managed additional work-study students to meet lab material and solution needs, and organized lab inventory Head of Lab Safety Managed day-to-day safety tasks like waste removal, lab cleanliness, and operations, and was responsible for formal and informal lab inspections and audits Undergraduate Mentor Mentored ten Northeastern University undergraduates and five summer researchers by developing projects and organizing experiments personalized to each student with the goal of having each student produce results that contribute to publications

Mentored five students through the National Science Foundation Research Experiences for Undergratuate (NSF-REU) students during a full time intensive summer research program. Managed projects, and taught tacit laboratory skills to accomplish research goals and meet experiment deadlines. Developed Individual Research Plans to achieve long term academic and career goals. Served on several panels for the program on how to apply to and succeed in graduate school.

Taught four laboratory sections of Principles of Biology. Lectured at the start of each lab, managed the setup of experiments throughout the lab, ensured the safety of students, and graded lab reports.

Utilized breast cancer patients' antigens to identify unique tumor associated antigens against mutated cells. Assisted in cloning VH regions from patient samples into expression plasmids and transfection of HEK 293T cells to produce antibodies that were generated from patient antigens. Antibodies were then screened to identify the original antigen. Assisted with general lab maintenance including making solutions and maintaining cell cultures.

Assisted in generating cell lines that were independently knocked down for the Sec 8 and Sec 10 proteins of the exocyst by utilizing a retrovirus system in MDCK cells. Analyzed the GP135 protein by tracking it using a pulse-chase method and immunofluorescence. Maintained various cell lines including, GP2 293 HEK, SNAPGP135 and SNAPcrb MDCK cells. Supported laboratory operations through troubleshooting and maintaining key equipment such as microscopes, incubators, and liquid nitrogen tanks