Changrui Xing

• Design, expression and purification - target proteins, mAbs, bispecifics, conjugates, and Fc fusions • Protein analytics - SEC, IEX, HIC, SDS-PAGE, WB, nanoDSF/DSC, AC-SINS, ELISA, BLI, SPR, LC-MS • HT-protein production and automation- Transcriptionally Active PCR (TAP) platform, Kingfisher, liquid handler • Antibody discovery campaigns- protein immunization, LNP immunization, phage display • Antibody discovery campaigns- humanization, epitope binning, binding kinetics etc. • Lead optimization (bispecific format, affinity tuning, immunogenicity, etc.) • Derisking manufacturability: Structural, biophysical, and stability evaluation of lead molecule panels • Co-leading Ampersand's therapeutic programs with counterparts from the Pioneering Medicines and Pfizer • Managing strategically outsourced activities at CROs

- Expression, purification and characterization of recombinant proteins including mitochondrial transporters and other membrane proteins - Development of functional assays to characterize purified mitochondrial transporters - Structure elucidation with cryo-EM

- Discovered the first cryo-EM structure of human cannabinoid receptor CB2-Gi complex - Developed and executed the protocols of CB2 construction, expression and purification - Screened and optimized CB2 constructs, CB2 ligands and thermostable mutations - Performed molecular docking, mutagenesis, competition binding assay, cAMP assay, GTPγS assay and BRET assay to study CB2 ligand binding and signaling mechanisms - Prepared various proteins and antibodies including CB2, CysLTRs, C5aR, PAR-1, p62, G protein, scFv and Fab for X-ray crystallography and cryo-EM studies

- Conducted virtual screening based on Cannabinoid Receptor 2 (CB2) homology model - Designed, synthesized and validated >40 novel CB2 selective compounds - Performed SAR studies

- Synthesized GSK3β irreversible inhibitors for Alzheimer's disease - Performed SAR studies to optimize the lead compound

-Participated in high-throughput screening assays to facilitate anti-HBV drug discovery process -Received training in basic experiments such as PCR, Southern Blot, Western Blot etc.

- Qualitative and quantitative analysis of >20 drugs on the market for the Quality Control - Received training in HPLC and GC