Brian Golbourn, Ph.D

• Collaborate across a multidisciplinary team to drive discovery biology projects to investigate lead targets. • Conceive and carry out biochemical and cellular-based assays designed to facilitate the development of lead compounds and medicinal chemistry campaigns. • Utilize shRNA and CRISPR-based genetic perturbation technologies for target validation studies

• Conceived and generated a novel mouse model of histone mutant pediatric gliomas. • Developed custom cell culture protocols to facilitate high-throughput amino acid dropout, genetic and drug screening applications for primary neural stem cell culture • Identified a sensitivity to methionine withdrawal in histone mutant tumors using an isogenic histone mutant mouse • model and further validated a targetable protein responsible for this methionine dependency. • Trained and provided mentorship to an 8-member research team comprised of technicians, undergraduate, graduate, and medical students. • Forged collaborations with academic groups and cores to develop assays to study the interplay between epigenetics and metabolism (LC/MS detection of SAM/SAH, ChIP-sequencing of histone modifications) • Wrote manuscripts published in peer-reviewed journals and presented at institutional seminars and meetings.

• Led a team of interns to develop and refine the formulation of the lead product, a gentle cleaning product that can • Effectively remove peanut allergens from all surfaces. • Drafted and finalized patents pertaining to the lead product and related technologies. • Devised and conducted stress tests to evaluate product stability and performance. • Sourced and negotiated supplies for batch test production and small-scale prototypes.

• Conceived and developed several animal models of a rare type of pediatric brain cancer by utilizing Cre-lox recombinase strains to target early neural precursor cells in developing embryos. • Combined bioinformatic analyses with rigorous screening technologies (genetic & pharmacological) to identify key vulnerabilities in disease models. • Wrote manuscripts published in peer reviewed journals and presented in international conferences and meetings.

• Provided acedemic support to undergraduate students in life sciences and chemistry disciplines. • Conducted tutorial sessions and one-on-one tutoring with undergraduate students. • Organized academic functions to facilitate student-faculty networking

Research focused on studying the role of the non-receptor kinase Fer in the pathogenesis breast and prostate cancer.

Prepared lectures on current course material, reviewed tutorial questions in class and prepared and administered quarterly tests