bill blake

Repeatedly generated confirmed targeted (knock-out/knock-in) mouse embryonic stem cell clones for 1) production of genetically modified mice and for 2) production of specific in vitro differentiated cell types Established and/or utilized protocols for in vitro differentiation of mouse embryonic stem cells to generate specific cell types such as macrophages, neurons, and cardiomyocytes to enhance the drug discovery process

Established and utilized human hematopoietic stem cells to assess compound effects on the process of proliferation and differentiation of purified Cd34+ cell populations to enucleated red blood cells Utilized mouse embryonic stem cell model of neuronal differentiation to assess ability of various agents to enhance population of dopaminergic neurons

Established and utilized primary hepatocyte models (genetically modified mouse, monkey) to examine effects of various agents on hepatic cholesterol/ lipoprotein metabolism Established and utilized rat pup cerebellar granule neuron model to assess efficacy/potency of compounds on phosphorylation state of Tau

Established primary rat hepatocyte model to assess PUFA/compound effects on de novo fatty acid synthesis and demonstrated PUFA inhibition of fatty acid synthase transcription Assessed efficacy/potency of anabolic agents in vivo utilizing hypohysectomized rodent model

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