Bijeta Prasai

1. Built the company's surface biology capability from scratch, establishing HTS-ready workflows to evaluate membrane protein expression, localization, and internalization for siRNA and antibody platforms. 2. Identified novel integrin-targeting small molecules to advance siRNA-conjugated delivery platforms. 3. Evaluated diverse primary cell types to characterize internalization and trafficking of fluorescently labeled compounds across unique integrin expression profiles. 4. Developed automated tissue and cell-quantification pipelines in QuPath, substantially increasing analysis throughput and reproducibility. 5. Collaborated with CROs to establish multiplex IF/IHC biomarker assays supporting fibrosis and oncology programs. 6. Provided specialized imaging, integrin profiling, and quantitative analysis expertise to cross-functional teams spanning fibrosis, DMD, and oncology programs. 7. Mentored and supervised Research Associates, fostering a culture of scientific rigor and technical growth within the HTS and imaging team.

1. Managed sales and marketing for Biological Research, Clinical and Educational accounts in East-Bay California region. 2. Consulted users about core and advanced systems for microscope selection in collaboration with research imaging and confocal specialists 3. Demonstrated, trained and provided post-sale technical support and troubleshot microscope issues 4. Established a core rep presence as a reliable microscopy resource for users in the East-Bay area.

Develop EM compatible and targetable protein labeling method, and investigate the precise location of proteins in single trafficking vesicles in 3D using CLEM and electron tomography Prasai, B., Haber, G.J., Strub, MP. et al. The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Nat Commun 12, 3970 (2021).

Used advanced imaging techniques including super-resolution fluorescence light and platinum replica transmission electron microscopy for nanoscale localization of proteins in sub-cellular organelles of secretory cells.

Used monolayer cancer cells and tumor mimics (3D-cell culture) to evaluate activatable fluorescent imaging agents with spectral properties ranging from ultraviolet to near-infrared window of electromagnetic spectrum. Devised and set up a 3D-spheroid (tumor mimics) system, formulated and optimized enzyme kinetic assay, IHC/IF and imaging assays for protein expression and function studies .