Bijal K.

- Executed GLP and GMP flow cytometry assay of CAR-T cells, immune cells, and mesenchymal stem cells for pharmaceutical clinical studies— - Performed wet-lab cell biology research, using methods including multi-color flow cytometry, enzyme-linked immunosorbent assay (ELISA), and aseptic maintenance of primary cell lines - Conducted assay development to transfer client protocol for in-house testing —- Conducted FDA Biologics License Application (BLA) validation runs —-Primarily performed flow cytometry assays using BD Canto II (BD Diva software) and BD Calibur (CellQuest Pro software) flow cytometers - Responsible for daily and monthly maintenance of all flow cytometers and other lab equipment —- Responsible for sample tracking via Laboratory Information Management System (LIMS)

- Executing bench research to discover biomarkers and methods of identify and isolate circulating tumor material from peripheral blood and bone marrow of breast and pancreatic cancer patients. - Worked on clinical research study processing samples from metastatic breast cancer patients - Maintain 6-7 mammalian breast cancer cell lines for various assay development projects - Assay development testing biomarker on CTC and PBMCs through multicolor flow cytometry on BD FACSVerse (up to 6 colors) - Processed and stored blood samples for plasma, buffy coat, and serum - Isolation of Peripheral Blood Mononuclear Cells (PBMCs) and CTCs from blood sample through density gradient separation (Ficoll) - Processed blood and bone marrow samples on the CellSearchTM platform in order to detect CTCs and DTCs - Assay development and validation of research biomarker antibodies on CellSearchTM platform in collaboration with Janssen - Assay development for different methods of CTC detection and isolation using normal donor blood with spiked variety of breast cancer tumor cell lines on flow cytometry and DEPArray

Research project title: Lipid Droplet Morphology in SR proteins and genes in the sex determination pathway of Drosophila melanogaster. - Collaborated with Widener Professors Dr. Alexis Nagengast and Dr. Hemlata Mistry - Research conducted during Widener University Summer Research Program, Summer 2012 & Summer 2013 and Academic years 2012 – 2013 & 2013 - 2014 - Used various techniques to observe the effect of knocking-down SR protein and genes of the sex-determination pathway on triglyceride levels and lipid droplet morphology - Dissection of D. melanogaster, Fat Body Staining, Triglyceride Assay, BSA Protein Assay

Research project title: Digestive Characteristics Of The Giant Jumping Stick Stiphra Sp. (Orthoptera: Proscopiidae) From Peru and Extatosoma tiaratum - Collaborated with Widener Professor Dr. Madigosky and 2 fellow students - Used bomb calorimetry to determine the determine the calorie intake of two different Arthropods

Minors in Biochemistry and Chemistry